大鼠急性肠道损伤动物模型的建立  

Establishment of a rat model of acute intestinal injury

作　　者：邢锐[1] 马高峰[2] 杨晓强[2] 武金宝[2] 陈学清[2] 张振书[2] 

机构地区：[1]广东省第二人民医院重症监护科,广东省广州市510317 [2]南方医科大学南方医院消化科,广东省广州市510015

出　　处：《世界华人消化杂志》2007年第27期2918-2922,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金;No 30570839~~

摘　　要：目的:用三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)复制大鼠急性肠道损伤的动物模型.方法:SD大鼠64只随机分为制模组、制模对照组及正常对照组,分别用TNBS(乙醇稀释)、500 mL/L乙醇及生理水灌肠;观察各组大鼠制模后的粪便、精神状态、进食及存活情况;分别在第1、3、5、7及10天处死大鼠,取结肠组织,进一步观察肠道的大体病理变化和组织病理变化;再结合病理评分,总结大鼠TNBS制模后肠道病理改变的规律,评价该模型用于实验性肠道损伤研究的可行性.结果:TNBS制模组在制模后第1天即表现出明显的肠道稀便和血便,持续至实验结束;进食减少、懒动、畏寒,持续7-10 d后缓解;4只在制模后第7-9天死亡(4/34);制模后第1天即出现肠道病理改变,第5天出现急性肠道损伤,第7天病理改变最严重.制模对照组大鼠在制模后第1天部分出现稀便,持续1-2 d后消失;制模后第1天肠道出现轻度病理改变,3 d后病变减退;正常对照组大鼠未见异常,各组大鼠肠道病理评分与病变程度一致.结论:大鼠TNBS制模后早期即表现出肠道损伤,制模后第7天病理改变达到高峰,此后向慢性炎症转化:TNBS制模后5 d内可用于急性肠道损伤的实验性研究.AIM： To develop a model of acute intestinal injury in rats treated with 2, 4, 6-trinitrobenzene sulfonic acid （TNBS）. METHODS： Sixty-four Sprague-Dawley rats were randomly divided into three groups： model, mock model and control groups. TNBS （ethanol-diluted）, 500 mL/L ethanol and physiological saline were administered per rectum to each of the three groups, respectively. Feces, psychosis, appetite and mortality were observed in each group. Rats were killed after 1, 3, 5, 7 and 10 d, and the colon was removed for gross observation and histopathological evalu- ation. By combining the pathological scores, we summarized the rule of gut pathological change of rats after TNBS treatment and evaluated the feasibility of using the model in the study of intestinal injury. RESULTS： On the first day, rats in the model group had thin and bloody stools until the end of the experiment. During the study, the rats were lazy, chilled and had loss of appetite, The symptoms lasted for 7-10 d, and alleviated thereafter. Four rats died （4/34） during 7-9 d after TNBS treatment. Pathological changes in the intestine showed up on d 1, acute intestinal injury appeared on d 5, and reached a peak on d 7. In the mock model group, the rats had thin stools on d 1, which persisted for 1-2 d. Pathological changes were mild and vanished on d 3. In the control group, the rats looked normal and there was no pathological change. The pathological score reflected the pathological changes. CONCLUSION： Intestinal injury emerged at the initial stage and reached a peak 7 d after treatment with TNBS. After that, the pathological changes became chronic. The first 5 d after the rats were treated with TNBS they were suitable for studying acute intestinal injury.

关 键 词：肠道损伤 三硝基苯磺酸 动物模型 

分 类 号：R-332[医药卫生] R574