益生菌VSL# 3对Oxazolone小鼠结肠炎结肠黏膜中β防御素-2表达的影响  

Effects of probiotic bacteria VSL# 3 on the expression of β defensin 2 in Oxazolone induced murine colitis

作　　者：常玉英[1] 欧阳钦[2] 强鸥[3] 

机构地区：[1]四川省第四人民医院消化内科,四川成都610016 [2]四川大学华西医院消化内科 [3]四川大学华西医院人类疾病相关多肽研究室

出　　处：《西部医学》2007年第6期1015-1018,共4页Medical Journal of West China

基　　金：国家自然科学基金项目资助(No.3270606)

摘　　要：目的观察益生菌VSL#3对Oxazolone小鼠结肠炎结肠黏膜中β防御素-2表达的影响。方法建立Oxazolone小鼠结肠炎模型并分为VSL#3治疗组、SASP治疗组、治疗对照组。免疫组化方法、RT-PCR及Western blot检测各组结肠粘膜组织中β防御素-2的蛋白及mRNA的表达。结果免疫组化方法、RT-PCR检测及Western blot检测发现VSL#3治疗组小鼠结肠粘膜中β防御素-2的蛋白及mRNA的表达低于治疗对照组,与SASP治疗组比较差异没有显著性。结论VSL#3对Oxazolone诱导的小鼠结肠炎有治疗作用,其作用机制可能与下调β防御素-2的表达有关。Objective To investigate the effects of probiotic bacteria VSL#3 on the expression of βdefensin 2 in colonic mucosa of mice with oxazolone-induced murine colitis. Methods The colitis model was established. The healthy female Balb/c mice of 6 to 8 weeks were divided into three groups ： VSL # 3 treatment group, SASP treatment group and control group. The expression ofβdefensin 2 in colonic mucosa were studied by immunohistochemistry assay, RT-PCR and Western blot analysis in three groups. Results The expression of β defensin 2 in colonic mucosa in VSL 3 treatment group was significantly lower than that in control group （P〈0.05）; there was no significant differences between VSL # 3 group and SASP group （P〉0.05）. The data measured by RT-PCR showed the expression of β defensin-2 mRNA in VSL # 3 treatment group was significantly lower than that in control group （P〈0.05）. There were no significant differences in VSL#3 and SASP groups （P〉0.05）. The expression of βdefensin 2 proteins in VSL # 3 treatment group by Western blot analysis was significantly lower than that in control group （P〈0.05）. There was no significant difference between VSL # 3 and SASP groups for prevention and treatment. Conclusion VSL # 3 is effective in the treatment of oxazolone induced colitis, which may be correlated to down regulation of the expression of βdefensin 2.

关 键 词：益生菌 结肠炎 防御素 

分 类 号：R363.14[医药卫生—病理学]