用新型分子模版吸附树脂从发酵液中分离红霉素的研究  

Study on the Separation of Erythromycin from Fermentation Liquid by New Absorption Resin

作　　者：鲁力[1] 王亚宁[2] 熊春华[3] 余蓉[1] 王槐三[2] 

机构地区：[1]四川大学华西药学院,成都610065 [2]四川大学国家生物医学材料工程中心,成都610065 [3]浙江工商大学化学系,杭州330306

出　　处：《四川农业大学学报》2007年第3期328-331,共4页Journal of Sichuan Agricultural University

基　　金：科技部科技型中小企业创新基金(06C26215100476);浙江省科技厅科技计划资助项目(2005C33043)。

摘　　要：采用新型分子模版吸附树脂动态吸附、丙酮解吸、水稀释结晶和丙酮重结晶等流程，从红色链霉菌发酵滤清液中分离红霉素，研究影响吸附分离效率的各种因素，并与现行醋酸丁酯萃取生产流程比较。结果显示，改性LX-18树脂对红霉素的吸附量达130ku／mL，对发酵液中色素的选择分离率〉95％，红霉素浓度120～150ku／mL的丙酮解吸液采用水稀释结晶，再用丙酮重结晶，即获得各项理化指标达药典标准的红霉素。结晶母液须2次吸附回收，红霉素总收率为60％～62％。本工艺具有简便、分离成本较低和减轻废水污染的特点。Useing neotype resin development-absorbing, acetone-desorption, water diluting to crystallize and acetone-recrystallisation separate the Erythromycin from red streptomycete fermentation scrubber liquid. Study all kinds of influence factors and compare with currently butylacetate-abstraction production flow-sheet. The result shows that the new reshaping resin LX- 18 does so well in absorbing the Erythromycin, its adsorption quantity reach 130 ku/mL and the separation for pigmentosus 95%, the Erythromycin concentration by 120N 150 ku/mL of acetone-desorption liquid use water diluting to crystallize, then acetone-recrystallisation. After that, it＇s all physical and chemical indicatrix can gain the pharmacopoeia standard. The crystal mother liquid need to twice adsorbing-recover, and the total yield of Erythromycin can reach 60 % -62 %. This separation method possesses simple process, high efficiency, low cost and only mild pollution wastewater.

关 键 词：红霉素 树脂吸附 发酵液 

分 类 号：TQ465.5[化学工程—制药化工] Q647.3[生物学—生物物理学]