供体NKT细胞输注促进小鼠皮肤移植耐受及其机理的研究  

Effect and Mechanisms of Transplantation Tolerance Induced by Donor Natural Killer T Cells(NKT) Infused Intravenously

作　　者：马群[1] 李春霞[1] 明建扩[1] 

机构地区：[1]济宁医学院免疫学教研室

出　　处：《济宁医学院学报》2007年第4期290-293,共4页Journal of Jining Medical University

摘　　要：目的探讨供体NKT细胞静脉输注诱导移植耐受的作用及其机制。方法C57BL/6和BALB/c小鼠分别为供体和受体,建立皮肤移植模型。对照组:术后注射生理盐水;NKT组:术前静脉注射供体鼠NKT细胞(5×106个/只),术后腹腔注射生理盐水;CsA组:术后腹腔注射环孢素(CsA)(4mg/kg);NKT+CsA组:手术前1日静脉注射供体鼠NKT细胞(5×106个/只),术后腹腔注射CsA(4mg/kg)。术后逐日观察移植皮肤存活情况及小鼠一般状况;术后14天对植皮BALB/c小鼠做混合淋巴细胞反应(mixed lymphocyte reaction,MLR)、迟发型超敏反应(delayed type hypersensitivity,DTH)等确定耐受的状态;并通过过继转移实验及脾细胞中细胞因子mRNA表达情况,进一步探讨耐受形成的机制。结果采用此诱导方案,移植皮片存活时间明显延长,耐受可被过继转移,Th2型细胞因子IL-10、IL-4在耐受小鼠中明显升高。结论供体NKT细胞静脉输注能诱导异基因小鼠皮肤移植耐受,抑制细胞、Th1/Th2偏移在耐受中起了一定作用。Objective Abstract Objective To investigate the effect and mechanisms of transplantation tolerance induced by donor natural killer T cells （NKT） infused intravenously. Methods Skin of C57BL/6 mice was transplanted to BALB/c mice. Control group：normal saline was given;NKT group： BALB/c mice were given an intravenous infusion of （5 × 10^6） donor NKT before transplantation and Sodium Chloride were give after transplantation; CsA group： Ciclosporin （CsA） （4mg/kg） were given after transplantation; NKT ＋ CsA group： NKT were given before transplantation and CsA were given after transplantation. Survival condition of grafted skin and general condition of mice were observed daily; 14 days after induction of tolerance the MLR and DTH of tolerance BALB/c mice were examined to confirm the tolerance status. Adoptive transfer assay and cytokine rnRNA expression in tolerant mice were performed to further explore tolerance mechanisms. Results Long - lasting skin allograft tolerance was achieved and the tolerance could be adoptively transferred. The levels of IL- 10 and IL- 4 mRNA in tolerant mice were much higher than those in the control mice. Conclusion Skin allograft tolerance of BALB/c mice could be induced by the infusion of donor NKT. Suppressor cells and deviation of Th1/ Th2 cytokines were involved in the process of tolerance.

关 键 词：NKT细胞 同种异基因移植 免疫耐受 IL-10/IL-4 

分 类 号：R57[医药卫生—消化系统]