肾癌组织中RASSF1A和BLU基因的异常甲基化  

Abnormal methylation of RASSF1A and BLU genes in renal carcinoma

作　　者：段建敏[1] 李智 赵敏 张剑英 彭波[1] 黄建华[1] 

机构地区：[1]同济大学附属第十人民医院泌尿外科,上海200072 [2]第十人民医院检验科

出　　处：《第二军医大学学报》2007年第10期1068-1071,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30270522);上海市科委基金(054119604).~~

摘　　要：目的:观察肾癌组织中RASSF1A和BLU基因启动子甲基化状态,探讨二者在肾癌发生中的可能作用。方法:采用甲基化特异性PCR(MSP)技术检测26例肾癌组织和相应癌旁组织中RASSF1A和BLU基因启动子甲基化状态,分析二者检测结果。结果:肾癌组织中17例(65.4%)RASSF1A基因异常甲基化表达,相应癌旁组织中无RASSF1A基因异常甲基化表达;11例(42.3%)肾癌组织中BLU基因高甲基化,相应癌周组织中未发现BLU基因高甲基化。结论:肾癌组织中RASSF1A和BLU基因启动子高度甲基化,表明RASSF1A和BLU基因甲基化可能与肾癌的发生相关。Objective：To investigate the methylation status of the promoters of RASSF1A and BLU genes in the renal carcinoma tissues and to assess their roles in the tumorigenesis of renal carcinoma. Methods： Methylation specific PCR （MSP） method was used to examine the methylation status of the promoters of RASSF1A and BLU genes in the renal carcinoma tissues of 26 patients with renal carcinoma and their corresponding adjacent normal tissues; and the results were analyzed. Results： We found that 17 （65.4% ） of the renal carcinoma tissues had abnormal methylation of RASSF1A gene, and there was no abnormal methylation of RASSF1A in the adjacent normal tissues. Eleven（42.3% ） of 26 renal cancer tissues had hypermethylation of BLU gene, and there was no hypermethylation of BLU gene in the corresponding adjacent tissues. Conclusion： Hypermethylation of RASSF1A and BLU genes is present in the renal carcinoma tissues, suggesting the hypermethylation of RASSF1A and BLU might be associated with the tumorigenesis of renal carcinoma.

关 键 词：肾肿瘤 基因 肿瘤抑制 DNA甲基化 RASSF1A BLU 

分 类 号：R737.11[医药卫生—肿瘤]