血管活性肠肽VIP对人结肠上皮细胞系HT29细胞AQP8表达调节的研究  

Regulation of AQP8 expression in HT29 cells by VIP

作　　者：冯现景 王俊平[1] 贾泽明[2] 

机构地区：[1]山西省人民医院消化科,山西太原030012 [2]卫生部肝胆肠外科研究中心,湖南长沙410008

出　　处：《中国现代医学杂志》2007年第19期2345-2348,2353,共5页China Journal of Modern Medicine

摘　　要：目的检测在体外培养的人结肠上皮细胞AQP8的表达情况,并研究VIP对其表达的调节作用,探讨肠道水代谢的分子生物学机制及其调节机制。方法采用免疫细胞化学染色和荧光定量逆转录聚合酶链反应(Fluorogenic probe quantitative reverse transcription-polymerase chain reaction,FQ-RT-PCR)的方法对在VIP作用下的人结肠上皮细胞AQP8蛋白和mRNA的表达进行定量分析研究。结果在人结肠上皮细胞系HT-29细胞中AQP8 mRNA和蛋白阳性表达。VIP诱导3h组HT-29细胞AQP8 mRNA的表达高于对照组和VIP诱导6、12和24h组(P<0.001)。VIP诱导3h组HT-29细胞AQP8蛋白的表达高于对照组和VIP诱导6、12和24h组(P<0.001)。结论AQP8主要在人结肠细胞膜丰富表达,胞浆内也有少量表达;VIP作用下的HT-29细胞AQP8 mRNA和蛋白的表达均增加,提示AQP可能是VIP作用的靶分子之一;VIP对AQP8的表达是短期快速调节。[Objective] To investigate the expression of AQP8 in a human colonic epithelial cells line, HT-29, and to characterize the regulation of AQP8 expression by VIP. To explore the molecular biology mechanisms of intestinal water metabolism and the regulatory mechanisms, and to provide the theory basis for the clinical treatment of water metabolism disorder correlation intestinal disease. [Method] Human colonic epithelial cells, HT-29, were incubated with VIP. The expression of AQP8 mRNA and the protein was determined by Fluorogenic probe quantitative reverse transcription-polymerase chain reaction, （FQ-RT-PCR） and Immunocytochemical, respectively. [Results] AQP8 mRNA and protein expresed in the human colonic epidermis cell line, HT-29 cell. The expression level of AQP8 mRNA at 24 h after addition of VIP was significantly higher than that of other time groups and the control （P 〈0.001）. The expression level of AQP8 protein at 3 h after addition of VIP was significantly higher than that of other time groups and the control （P 〈0.001）. [Conclusion] AQP8 is expressed in the human colonic surface epithelial cell abundantly, whereas less extent in intracytoplasm. AQP8 mRNA and protein expression in HT-29 cells was upregulated by VIP Therefore we can conclude that AQP may be one of the target molecules in VIP causing diarrhea, The regulation of AQP8 expression by VIP is short-term.

关 键 词：血管活性肠肽 水通道蛋白8 HT-29细胞 

分 类 号：R363[医药卫生—病理学]