免疫调节性寡聚脱氧核苷酸筛选方法的建立、优化和应用  

Establishment,optimization and application of a screening method for immunoregulatory oligodeoxynucleotides

作　　者：胡大利[1] 张雪松[2] 王华颖[3] 孙然[1] 任继玲[1] 王丽颖[3] 于永利[1] 

机构地区：[1]吉林大学基础医学院免疫学教研室 [2]吉林省血液中心,吉林长春130021 [3]吉林大学基础医学院分子生物学教研室

出　　处：《细胞与分子免疫学杂志》2007年第11期1017-1020,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金海外杰出青年基金资助(30328010)

摘　　要：目的:建立一套合理而便捷的实验体系,为开发新型免疫调节性寡聚脱氧核苷酸(ODN)提供筛选方法。方法:利用含有CpG基序的免疫刺激性寡聚脱氧核苷酸(CpG ODN)作为刺激物,将人外周血单个核细胞(PBMC)作为研究对象,分别以细胞的增殖程度和刺激上清的抗病毒活性作为检测指标,优化各项实验条件,综合评定寡聚脱氧核苷酸的免疫调节活性。结果:成功建立了由阳性免疫调节性ODNA151抑制CpG ODN诱导的人PBMC增殖及抗病毒活性的筛选方法。结论:免疫调节性ODN筛选方法的成功建立,为下一步研究开发新型免疫调节性ODN奠定了基础。AIM： To establish a set of reasonable and convenient experimental system to provide a screening method for the development of novel immunoregulatory oligodeoxynucleotides. METHODS： The human PBMCs were stimulated by CpG ODN and/or immunoregulatory ODN. The cell proliferation and anti-viral activity of the supernatant induced by CpG ODN were examined by thymidine incorporation and anti-viral bioassay to evaluate the immunoregulatory activity of candidate ODN. The experimental conditions were also optimized. RESULTS： A screening method on which A151, a positive immunoregulatory ODN, inhibited the proliferation and anti-viral activity of the CpG ODN-induced human PBMCs was successfully established. CONCLUSION： The successful establishment of CpG ODN based screening method lays the foundations for further development of novel immunoregulatory oligodeoxynucleotides.

关 键 词：免疫调节 寡聚脱氧核苷酸 CPG ODN 人外周血单个核细胞 

分 类 号：R392.11[医药卫生—免疫学]