染料木素对人子宫内膜癌细胞系HEC-1B体内外增殖抑制作用的研究  

Study on antiproliferative effects of genistein on human endometrial cancer cell line HEC-1B in vivo and in vitro

作　　者：沈国栋[1] 凌斌[1] 周颖[1] 冯定庆[1] 程志祥[1] 陈峥峥[1] 姚凤球[1] 高婷[1] 石永云[1] 王梅梅[1] 

机构地区：[1]安徽医科大学附属省立医院安徽省分子医学重点实验室,合肥230001

出　　处：《中国临床保健杂志》2007年第6期614-617,共4页Chinese Journal of Clinical Healthcare

基　　金：安徽省科技攻关计划项目(06013124B)

摘　　要：目的研究染料木素（genistein，GEN）对人子宫内膜癌细胞系HEC-1B细胞体内外增殖的抑制作用，探讨其抗癌作用的机制。方法采用四甲基偶氮唑蓝比色分析（MTT）、病理组织切片HE染色等方法，分别检测体内外GEN对人子宫内膜癌细胞系HEC-1B增殖的抑制效果。结果体外实验：GEN浓度为0—25μmol／L时，对HEC-1B细胞有明显促进增殖作用（P〈0．05），随着GEN浓度升高至50μmol／L时，对HEC．1B细胞则呈现抑制作用，当GEN浓度为100μmol／L时，则显示了明显的生长抑制作用（P〈0．05），其抑制率达到55％。体内实验：人子宫内膜癌裸鼠模型经100μmol／L染料木素处理后体内平均瘤质量明显减小（P〈0．05），抑瘤率可达39％，且处理后肿瘤组织微血管数量减少，部分区域坏死明显。结论染料木素能够明显抑制人子宫内膜癌细胞及其移植瘤的生长，其机理可能为通过和雌激素竞争结合雌激素受体，减轻雌激素促细胞增殖作用和抑制肿瘤血管生成导致肿瘤细胞坏死，从而发挥抗肿瘤作用。Objective The inhibitive effects of genistein on the proliferation of endometrial cancer cell line HEC-1B in vitro and in vivo were investigated, and its anticancer mechanism were explored. Methods MTT, HE stain of histopathologic slides and others Were used to assay the inhibitive effects of genistein on the proliferation of endometfial cancer cell line HEC-1B in vitro and in vivo, respectively. Results In vitro, genistein ranged from 0 to 25μmol/L in the medium had a marked promotion on the proliferation of HEC-1B cells （P 〈 0. 05 ）. However,it exerted an inhibitive action on the cancer cells at the concentration of 50μmol/L, and a significant antiproliferative effect occurred at 100μmol/L with a fifty-five percent of inhibition rate （P 〈 0.05 ）. In vivo, after the treatment of 100μmol/L genistein, the average tumor weight of nude mice injected by HEC-1B cells remarkably reduced（P 〈 0. 05）,with its inhibition rate by thirty-nine percent, the decreased number of microvessel and obvious necrosis was found in tumor tissues under a microscope. Conclusions Genistein could effectively inhibit the proliferation of endometfial cancer cell line HEC-1B and its induced tumors in nude mice, and the anticancer mechanism was presumed to antagonize estrogen to bind estrogen receptors, them the proliferative and tumor angiofenesis effects of estrogen were inhibited,which result in the necrosis of the cells finally.

关 键 词：子宫内膜肿瘤 染料木黄酮 细胞系 肿瘤 雌激素替代疗法 模型 动物 

分 类 号：R737.33[医药卫生—肿瘤]