p21活化激酶1基因在大肠癌细胞中的表达及意义  

Significance and mechanisms of overexpression of PAK1 gene in colorectal carcinoma cells

作　　者：武金宝[1] 南清振[1] 张绍荣[1] 张弟[1] 张振书[1] 宋于刚[1] 

机构地区：[1]南方医科大学南方医院,广东广州510515

出　　处：《山东医药》2007年第29期1-3,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30171053)。

摘　　要：目的探讨p21活化激酶l(PAK1)基因在大肠癌细胞中的表达及意义。方法运用免疫印迹技术检测PAK1基因在8株不同转移和恶性潜能大肠癌细胞(LoVo、SW480、SW620、SW1116、HT29 HCT116和CO-LO320细胞)中的表达。结果与HCT116、HT29、SW480、SW1116和LST细胞相比,PAK1在LoVo、COLO320和SW620大肠癌细胞中蛋白表达明显增加,而HT29和SW480细胞中的PAK1蛋白表达亦强于SW1116和LST细胞,但PAK1在SW1116和LST细胞几乎无表达。结论PAK1蛋白过度表达可促进大肠癌的发生、发展,且可能与大肠癌的恶性生物学表型密切相关。[ Objective] To investigate the significance and mechanisms of expression of p21- activated kinase 1 （PAK1） in colorectal carcinoma cells in vitro. [ Methods] PAK1 expression was detected in the 8 established cell lines of colorectal carcinoma with different metastasis and malignant potentials by western blot. [ Results ] The expression of PAKI was significantly higher in LoVo, COLO320 and SW620 cell lines compared with HCT116, HT29, SW480, SW1116 and LST cells, the expression of PAK1 protein in HT29 and SW480 cell lines was higher than that in SW1116 and LST cells. Furthermore ,the expression of PAK1 in SW1116 and LST cell lines was almost lost. [ Conclusion ] Overexpression of PAK1 protein may be involved in the tumorigenesis and progression of colorectal carcinoma and it is associated closely with the malignant phenotype of colorectal carcinoma.

关 键 词：大肠肿瘤 大肠癌 PAK1基因 免疫印迹 

分 类 号：R735.3[医药卫生—肿瘤]