两株滇产广义美味牛肝菌的分离培养及其分子鉴定  

Cultivation and Molecular Identification of Two Bolete Strains Isolated from Boletus edulis Complex of Yunnan,China

作　　者：王海坤[1,2] 李涛[1] 赵丹丹[3] 李凌飞[1] 沙涛[1] 赵之伟[1] 

机构地区：[1]云南大学,生物资源保护与利用国家重点实验室培育基地,微生物多样性可持续利用教育部重点实验室,云南昆明650091 [2]昆明师范高等专科学校,云南昆明650205 [3]云南省农业科学院园艺作物研究所

出　　处：《云南植物研究》2007年第5期559-562,共4页Acta Botanica Yunnanica

基　　金：云南省自然科学基金(2005C003M)资助课题

摘　　要：采用组织分离法从广义美味牛肝菌子实体分离获得2株稳定的菌株,初步研究了两菌株的分离和培养条件。用ITS序列分析,对分离菌株进行了分子鉴定。基于ITS序列构建的系统树表明两株菌属于美味牛肝菌复合群,并与夏生牛肝菌Boletus aestivalis(Paul.)Fr.有较近的亲缘关系。Two bolete strains were obtained from fruiting bodies by tissue isolation and culture methods. Conditions for isolation and cultivation of the two strains were studied. Molecular identification was carried out based on sequence analysis of the internal transcribed spacers （ITS1-5.8S-ITS2） of the strains. Neighbour-joining analysis showed that the two isolates belong to the Boletus edulis complex and have a close phylogenetic relationship to B. aestivalis.

关 键 词：美味牛肝菌 分离培养 分子鉴定 

分 类 号：S646[农业科学—蔬菜学]