联用色谱和化学计量学方法分析赤芍挥发性成分  

Determination of volatile chemical components of Radix Paeoniae Rubra by GC-MS and HELP method

作　　者：李国辉[1] 兰正刚[1] 李晓如[1] 

机构地区：[1]中南大学化学化工学院

出　　处：《中南大学学报（自然科学版）》2007年第1期89-92,共4页Journal of Central South University:Science and Technology

基　　金：国家自然科学基金资助项目(20235020)

摘　　要：采用气相色谱-质谱(GC-MS)检测,通过直观推导式演进特征投影法(HELP)对二维色谱／质谱数据进行解析,得到各组分的纯色谱曲线和质谱,从而实现对赤芍挥发性成分的定性和定量分析。根据色谱保留时间和质谱,在质谱库中进行相似检索,实现对组分的定性分析,然后用归一化法进行定量分析。研究结果表明:从赤芍挥发性成分中共分辨出57个色谱峰,通过质谱库对分辨的纯组分进行检索,鉴定其中36个成分,占挥发性成分总含量的95．21％;赤芍主要挥发成分为水杨醛、正十六碳酸和(Z,Z)-9,12-十八碳二烯酸。The volatile chemical components of radix paeoniae rubra （RPR） were analyzed qualitatively and quantitatively by gas chromatography-mass spectrometry （GC-MS）, heuristic evolving latent projections （HELP） method and normalization method. According to retention time of chromatography and mass spectra, similarity searches were conducted in the MS database, and the chemical components of volatile oil in RPR were analyzed qualitatively, and then quantitative calculation was carried out using normalization method. Thirty-six of volatile chemical components of RPR were determined, accounting for 95.21% of total contents of volatile chemical components of RPR. The mail volatile chemical components of RPR are （Z, Z）-9, 12-octadecadienoic acid, n-hexadecanoic acid and 2-hydroxy- benzaldehyde.

关 键 词：赤芍 挥发性成分 GC/MS HELP法 

分 类 号：O657[理学—分析化学]