溃疡性结肠炎中信号转导和转录活化因子6的活化表达及作用  

The role of the activation and expression of signal transducer and activator of transcription-6 in patients with ulcerative colitis

作　　者：庞艳华[1] 郝建宇[1] 张文杰[2] 郑长青[3] 

机构地区：[1]首都医科大学附属北京朝阳医院消化科,100020 [2]武汉大学中南医院医学科学研究中心 [3]中国医科大学附属第二院消化科

出　　处：《中华消化杂志》2007年第10期679-681,共3页Chinese Journal of Digestion

摘　　要：目的检测信号转导和转录活化因子6(STAT6)在溃疡性结肠炎(UC)中的表达,探讨其在UC发病中的作用机制。方法选择结肠镜诊断并经病理证实的UC患者30例,均为活动期患者,且未用激素及免疫抑制剂治疗。同时选择性别年龄相匹配的正常对照者30例。用Western印迹法分别检测标本核蛋白与胞质蛋白中磷酸化和非磷酸化STAT6的表达,同时用凝焦电泳迁移率改变分析法(EMSA)检测STAT6的DNA结合活性。结果Western印迹结果显示,在轻度UC患者,细胞核磷酸化STAT6表达低于细胞质,而在中、重度UC患者则胞核表达高于胞质。EMSA结果亦显示,UC组STAT6的DNA结合活性高于对照组,且随炎症程度加重而有逐渐增强的趋势。结论STAT6可能参与UC的发病过程并在UC的发病中起作用。Objective To investigate the pathogenesis of the expression and activation of signal transducers and activator of transcription（STAT） 6 in patients with ulcerative colitis（UC）. Methods Thirty patients with active UC（ diagnosed with colonoscopy with pathologicaly conformation）, who were not treated with steroids or immunomodulators, and 30 age-matched healthy controls were studied, The expressions of phosphorylated STAT6 and nonphosphorylated STAT6 in nucleus and cytoplasm were deterimined by Western blot. DNA binding activity of STAT6 was tested by electrophoresis mobility shift assay（EMSA）. Results The expression of phosphorylated STAT6 was higher in nucleus than that in cytoplasm in mild UC patients, but the expression was higher in cytoplasm than that in nucleus in moderate to sever UC patients（P〈 0.05）. The DNA binding activity of STAT6 in UC patients was increased. It is also associated with disease activity. Conclusion STAT6 may involve in pathogenesis of UC.

关 键 词：结肠炎 溃疡性 信号转导 反式激活因子类 

分 类 号：R574.62[医药卫生—消化系统]