长期口服家蚕生产的CTB-InsB融合蛋白对延缓NOD小鼠Ⅰ型糖尿病发作的研究  

Oral administration of CTB insulin B chain produced in silkworm delays the diabetes development in NOD mice

作　　者：周灵娟[1] 金勇丰[1] 

机构地区：[1]浙江大学生命科学学院生物化学研究所,杭州310058

出　　处：《四川大学学报（自然科学版）》2007年第5期1125-1128,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：教育部新世纪优秀人才基金(NCET-04-0531G50503)

摘　　要：为对霍乱毒素B亚基(the cholera toxin B subunit,CTB)联合胰岛素B链(insulin Bchain)表达的融合蛋白CTB-InsB长期口服延缓糖尿病发作的效果进行研究,将Ⅰ型糖尿病动物模型NOD小鼠随机分组,给予口服融合蛋白CTB-InsB后,检测尿糖、血糖,观测CTB-InsB对模型小鼠发病率的影响.试验表明与口服野生蚕血的对照组相比,每次口服融合蛋白CTB-InsB 50μg对NOD小鼠糖尿病的发作具有明显延缓作用(P<0.05),而100μg和10μg的剂量虽有下降趋势但是却差异不显著(P>0.05).An fusion protein that insulin B-chain peptide（InsB）was conjugated with the cholera toxin B subunit （CTB） was administered orally to NOD mice which could develop type Ⅰ diabetes spontaneously, in order to demonstrate the diabetes delay effect of CTB-InsB . The urine glucose then was observed, the blood glucose was detected and the effect of the fusion protein was analyzed. Compared with the control group which was fed with wild type silkworm blood lymph, this fusion protein at the dosage of 50 l,g per feeding can statistically efficaciously prevents the mice from the rapid development of diabetes （P 〈 0.05）. Whereas the administration of 100μg and 10μg per feeding appears not so distinctly to decrease the incidence of diabetes （P 〉 0.05）, although those dosages still show downtrend.

关 键 词：Ⅰ型糖尿病 NOD小鼠 CTB-InsB融合蛋白 口服耐受 旁观抑制 

分 类 号：R977[医药卫生—药品]