实时荧光定量RT-PCR检测肝细胞肝癌中B-myb的表达及其临床意义  

Expression and clinical significance of B-myb in hepatocellular carcinoma with the application of real time fluorescent quantitative RT-PCR

作　　者：吴华[1] 曹骥[1] 杨春[2] 李丽萍[2] 欧超[2] 潘南海[3] 段小娴[2] 李瑗[2] 苏建家[2] 岳惠芬[2] 

机构地区：[1]广西医科大学医学科学实验中心,南宁市530021 [2]广西医科大学附属肿瘤医院,南宁市530021 [3]广西医科大学,南宁市530021

出　　处：《广西医学》2007年第11期1663-1666,共4页Guangxi Medical Journal

基　　金：广西自然科学基金资助课题(桂科青0447050);广西留学回国人员科技活动择优资助课题(桂人函2005321)

摘　　要：目的建立B-myb mRNA表达量检测的实时荧光定量聚合酶链反应(RT-PCR)系统,检测肝细胞肝癌(HCC)中B-myb的表达并分析其临床意义。方法用实时荧光定量聚合酶链反应技术检测70例HCC患者癌组织、癌旁肝组织及18例正常肝组织中B-myb mRNA的表达情况。结果B-myb mRNA在肝癌组织(0.0375±0.0168)及癌旁肝组织(0.0353±0.0128)中的表达水平明显高于在正常肝组织(0.0265±0.0099)中的表达水平(P<0.05),而在肝癌组织及癌旁肝组织中的表达水平差异无统计学意义(P>0.05)。B-myb在人肝癌组织中的表达与临床分期、肝外转移及术后复发明显有关,而与门静脉癌栓、肿瘤个数、肿瘤直径、血清AFP水平及分化程度无明显关系。结论该方法克服了传统PCR只能定性检测而不能定量检测的缺点,为研究B-myb在HCC中的表达提供了定量方法。B-myb可能与肝癌的发生、发展有关。Objective To develop a real time fluorescent quantitative reverse transcriptase polymerase chain reaction （RT-PCR） system for determining the expression of B-myb mRNA in hepatoceUular carcinoma, and analyse its clinical significance. Methods The expression of B-myb mRNA in 70 HCC tissues and their adjacent liver tissues and 18 normal liver tissues was determined by real time fluorescent quantitative RT-PCR . Results The expression level of B-myb mRNA in the HCC tissue （0.0375±0.0168） and the adjacent liver tissue （0.0353 ±0. 0128 ） was markedly higher than that in the normal liver tissues （0.0265 ± 0.0099, P 〈 0.05 ）. The expression level of B-myb mRNA was not significantly different in the HCC tissue compared with that in the adjacent liver tissue （ P 〉 0.05）. The expression of B-myb in the HCC tissue significantly correlated with the clinical stage, the presence of extrahepatic metastasis, and the recurrence of tumor,but did not correlated with the portal vein tumor thrombus, the number of tumor, the diameter of tumor, the level of sertan alpha-fetoprotein （AFP） ,and the differentiation of tumor. Conclusion Real time fluorescent quantitative RT-PCR has an advantage of giving the quantitative expression of B-myb comparing to the traditional PCR which was only applied to the qualitative expression. The results suggest that B-myb may be related to the initiation and progression of HCC.

关 键 词：肝细胞肝癌 B-MYB 实时荧光定量聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]