毛细管电泳法快速测定五倍子中的没食子酸  

Rapid determination of gallic acid in galla chinensis by capillary electrophoresis

作　　者：翟海云[1] 杨冰仪[1] 黄庆华[1] 陈缵光[2] 

机构地区：[1]广东药学院,广州510224 [2]中山大学药学院,广州510089

出　　处：《分析试验室》2007年第11期35-37,共3页Chinese Journal of Analysis Laboratory

基　　金：广东省科学技术(2004B10401027);广东药学院博士启动基金项目资助

摘　　要：建立了毛细管电泳高频电导法测定没食子酸的方法。探讨了缓冲溶液、有机溶剂添加剂、分离电压等因素对分离检测的影响。在电泳介质为10.0mmol/L Tris-5.0 mmol/L H3BO3-15.0%C2H5OH,分离电压22.0 kV的优化条件下,5.5 min内即可实现没食子酸的分析,线性范围为3.00～100μg/mL,检出限为1.0μg/mL。成功地检测了五倍子中的没食子酸。A rapid method for the determination of gallic acid by capillary electrophoresis with high frequency conductivity detection was described. The key factors for separation and determination were studied and the optimum analysis conditions were obtained, in the buffer solution of 10.0 mmol/L Tris-5.0 mmol/L H3BO3-15.0% C2H5OH at the separation voltage of 22.0kV gallic acid could be separated and detected within 5.5min. Gallic acid showed good linear response in the range of 3.00- 100μg/mL, and the limit of detection was 1.0μg/mL. The method was used for analysis of gallic acid in galla chinensis satisfactorily.

关 键 词：毛细管电泳 高频电导检测 非接触式电导检测 五倍子 没食子酸 

分 类 号：O657.8[理学—分析化学]