苏云金芽胞杆菌伴胞晶体蛋白对植物寄生线虫生物测定方法的建立和高毒力菌株的筛选  

Establishment of Bioassay Procedure with Bacillus thuringiesis Crystal Protein against Plant-parasitic Nematodes and Screening of Strains with High Toxicity

作　　者：余子全[1] 王乾兰[1] 刘斌[1] 邹雪[1] 喻子牛[1] 孙明[1] 

机构地区：[1]华中农业大学生命科学技术学院农业微生物学国家重点实验室,武汉430070

出　　处：《农业生物技术学报》2007年第5期867-871,共5页Journal of Agricultural Biotechnology

基　　金：国家高技术研究与发展计划(863)项目(No.2006AA02Z174;No.20006AA10A212);国家重点基础研究发展规划(973)项目(No.2003CB114201)资助

摘　　要：建立了一套苏云金芽胞杆菌(Bacillus thuringiensis)伴胞晶体蛋白对植物寄生线虫的生物测定方法。利用该方法筛选出了10株对植物寄生线虫有毒力的菌株。SDS-PAGE显示这些菌株的伴胞晶体蛋白组成具有特异性和多样性。选取其中的1株高毒力菌株YBT-021,测定了对几种植物寄生线虫的毒力,其半致死浓度(LC50)分别为:北方根结线虫(Meloidogyne hapla)35.62μg/mL,斯氏短体线虫(Pratylenchus scribneri)75.65μg/mL,苎麻(Boehmeria nivea)上的一矮化线虫群体(Tylenchorhynchus sp.)94.31μg/mL和腐烂茎线虫(Ditylenchus destructor)215.21μg/mL。A bioassay procedure of Bacillus thuringiensis parasporal crystal protein against plant-parasitic nematodes was established. By this procedure, 10 strains with nematicidal activity were isolated. The paraspoml crystal proteins of 10 Bt sWains were determined by SDS-PAGE, and its profile demonstrated specificity and diversity. Among of these swains, swain YBT-021 showed high toxicity. Its toxicity against plant-parasitic nematodes, like Meloidogyne hapla, Pratylenchus scribneri, Tylenchorhynchus sp. and Ditylenchus destructor was assayed, with LC50 values at 35.62, 75.65, 94.31 and 215.21 μg/mL, respectively.

关 键 词：苏云金芽胞杆菌 伴胞晶体蛋白 生物测定 植物寄生线虫 

分 类 号：S188[农业科学—农业基础科学]