化疗对B-NHL患者外周血IgH基因重排的影响  

The influence of the chemotherapy on the IgH generearrangement in the peripheral blood of the B cell lymphoma patients

作　　者：李娟[1] 胡长路[1] 余元勋[2] 施姗姗[1] 

机构地区：[1]安徽医科大学附属省立医院肿瘤科,合肥230032 [2]安徽医学高等专科学校遗传中心,230601

出　　处：《临床肿瘤学杂志》2007年第10期740-742,共3页Chinese Clinical Oncology

基　　金：安徽省卫生厅"十.五"课题(2002B074)

摘　　要：目的:通过观察B细胞性非霍奇金淋巴瘤(B-NHL)患者化疗过程中IgH基因重排的阴转情况,了解IgH基因重排能否作为适时的肿瘤分子水平缓解指标。方法:对20例B-NHL的初治患者于化疗前及化疗达到部分缓解及完全缓解后应用多聚酶链反应(PCR)进行IgH基因重排检测;对10例非淋巴瘤的肿瘤患者在化疗前后行IgH基因重排检测;同期检测10例正常人。结果:20例初治B-NHL患者,化疗前18例检测到IgH基因单克隆型重排;化疗后7例达到完全缓解,6例达到部分缓解。18例阳性患者中,除1例化疗后达到完全缓解的患者IgH基因重排转阴外,余未变化。2例IgH基因单克隆型重排阴性的病例化疗后检测结果仍为阴性。对照组的肿瘤患者化疗前后外周血以及正常人外周血IgH基因单克隆重排均为阴性。结论:IgH基因单克隆型重排虽可以作为诊断B-NHL及检测微小残留病变的重要指标,但是肿瘤缓解后的短期内还不能作为适时的肿瘤分子水平缓解指标。Objective：To observe the IgH generearrangement in the peripheral blood of the B cell lymphoma patients before and after the chemotherapy to know if it will be the timely therapeutic index of tumor on molecular level. Methods： Using the polymerase chain reaction to test the IgH generearrangement of twenty B-NHL patients and ten tumor patients who were not lymphomatous before and after the chemotherapy, respectively; And ten health adults were tested as contral group. Results：Twenty patinets accepted fisrt chemotherapy were examed before the chemotherapy. Eighteen patients were positive of monoclonal IgH generearrangement. After the total chemotherapy ,7 patients achieved complete remission and 6 patients achieved partial remission. Only one patient who got CR was to negative. The index of the control group was negative. Conclusion： IgH generearrangement may be an important index to diagnose B-NHL and to detect MRD, but it may not be the timely therapeutic index of tumor on molecular level.

关 键 词：IGH基因重排 非霍奇金淋巴瘤 多聚酶链反应 化学治疗 

分 类 号：R730.53[医药卫生—肿瘤] R733.1[医药卫生—临床医学]