装载mIFN-γ基因的溶瘤腺病毒CNHK200的抗肝癌活性  

The payload of mIFN-γ gene enhances the antitumor activity of the oncolytic adenovirus CNHK200 in hepatocarcinoma

作　　者：陈洁[1] 达万明[2] 苏长青[3] 薛惠斌[3] 李林芳[3] 姜梨华[3] 吴红平[3] 钱其军[3] 

机构地区：[1]第二军医大学长海医院血液病科,上海200433 [2]北京解放军总医院血液病科,100853 [3]上海第二军医大学东方肝胆外科医院,200438

出　　处：《临床肿瘤学杂志》2007年第10期750-752,756,共4页Chinese Clinical Oncology

基　　金：国家自然科学基金国际合作重大项目(30120160823)

摘　　要：目的:研究鼠源γ-干扰素(mIFN-γ)基因插入溶瘤腺病毒CNHK200后,该病毒对不同的肝癌细胞系及其裸鼠移植瘤的增殖力及特异性杀伤作用。方法:用MTT法检测CNHK200-mIFN-γ在体外对人正常肝细胞L02、人肝癌细胞Hep3B、HepGⅡ的特异性杀伤作用;裸鼠体内试验观察CNHK200-mIFN-γ对肝癌Hep3B模型的抗肿瘤疗效。结果:CNHK200-mIFN-γ对正常人肝细胞L02无杀伤作用,但能特异性杀伤肝癌细胞,mIFN-γ的插入使病毒对肿瘤细胞的杀伤能力提高;动物体内,CNHK200-mIFN-γ具有明显的肿瘤生长抑制作用。结论:CNHK200-mIFN-γ可以高效率地杀死肝癌肿瘤细胞,而不杀伤正常细胞,可能具有良好的临床应用前景。Objective：To study the effect of mlFN-γ, insertion on the selective replication and cytotoxicity of a tumor-specific oncolytic adenovirus CNHK200 in hepatocarcinoma cells and in xenografts. Methods ：The cytotoxicity of CNHK200-mlFN-γ was examined by MTT colorimetric assay in normal and tumor cells. The antitumor activity of CNHK200-mlFN-γ, was observed with Hep3B models in nude mice. Results： CNHK200-mlFN-γ, could selectively kill tumor cell lines, and the antitumor activity of CNHK200-mlFN-γ, was also demonstrated with Hep3B models in nude mice. Conclusion： CNHK200-mlFN-γ could significantly kill hepatocarcinoma cells, but showed no effect on normal cells, indicating a splendid future of CNHK200-mlFN-γ, as a potential antitumor agent.

关 键 词：溶瘤腺病毒 CNHK200 mIFN-γ基因 基因治疗 

分 类 号：R735.7[医药卫生—肿瘤]