二价金属离子对博莱霉素作用淋巴细胞SCE的影响  

Effect of Catholytes on Bleomycin Treated Lymphocyte Sister Chromatid Exchanges (SCE)

作　　者：周白云[1] 宗传龙[2] 郭淼[2] 

机构地区：[1]泰山医学院医学检验系,山东泰安271016 [2]泰山医学院基础医学部,山东泰安271000

出　　处：《中国肿瘤》2007年第11期937-939,共3页China Cancer

基　　金：山东省泰山医学院资助:项目(编号424)

摘　　要：[目的]观察金属离子和离子络合剂对博莱霉素(BLM)作用淋巴细胞SCE的影响。[方法]在含有一定量BLM的人外周血淋巴细胞培养物中注入一定量的金属离子或离子络合剂,统计淋巴细胞的姐妹染色单体交换(SCE)率。[结果]终浓度为0.5mmol/L和1.0mmol/L的Fe2+、终浓度为2.0mmol/L和4.0mmol/L的Mg2+在BLM切割DNA上有明显的促进作用(P<0.05);终浓度为2.0mmol/L的Fe2+则有极其明显的促进作用(P<0.01);同为2.5mmol/L的EDTA和枸橼酸钠则有明显的拮抗作用(P<0.05)。[结论]一定量的Fe2+和Mg2+的存在对于BLM切割DNA的过程是必需的。[Purpose ] To investigate the effect of catholyte and its chelating agent on bleomycin treated lymphocyte sister chromatid exchanges （SCE）. [Method] SCE was measured after catholytes or chelating agents incubated with cultured human peripheral blood lymphocytes which had been treated with BLM. [Resultsl Both groups of ferrous chloride （0.5 or 1.0mmol/L） and magnesium chloride （2.0 or 4.0mmol/L） showed a significant difference with the control group in cutting lymphocyte DNA fragments （P〈0.05）. The group of ferrous chloride （2.0mmol/L） showed a remarkable significant difference with the control group （P〈0.01）. Both sodium citrate （SC） and EDTA （2.5mmol/L both） showed a significant difference compared to the control group （P〈0.05）. [Conclusions] Both ferrous ion and magnesium ion are necessary to process of bleomycin on lymphcyte DNA fragment cutting.

关 键 词：博莱霉素 姐妹染色单体交换 金属离子 

分 类 号：R73-361[医药卫生—肿瘤]