DNA结合抑制蛋白-1在结肠癌中的表达及其意义的研究  

Study on Expression of Inhibitors of DNA inding-l（Id-l）Protein in Human Colon Cancer and its Clinicopathological Significance.

作　　者：樊鸿炎[1] 薛迪强[1] 杜宏伟[1] 李素萍[1] 郝芳[1] 温红旭[1] 

机构地区：[1]甘肃省兰州市第二人民医院,730046

出　　处：《中华临床医学杂志》2007年第10期8-11,共4页Chinese Journal of Clinical Practical Medicine

摘　　要：目的探讨结肠癌中DNA结合抑制蛋白-1（Id—1）的表达与肿瘤细胞分化程度、浸润深度、淋巴结转移及TNM分期的关系。方法用免疫组化方法分别检测17例结肠腺瘤、16例不典型增生、52例结肠癌及J5例癌旁正常纽织中Id—1的表达。结果正常肠粘膜组织中Id-1蛋白偶有表达，在结肠腺瘤、不典型增生、结肠癌组织中的阳性表达率分别为29.4％、43．75％、73．1％，三者比较差别有统计学意义（P〈0．05）。分化程度低的结肠癌组织中Id．1的阳性表达率高于分化程度高者，两者比较差别有统计学意义（P〈0．05）；35例伴有淋巴结转移的肿瘤原发灶者29例（82．9％）表达Id-1，17例无淋巴结转移者9例表达Id-1（52．9％），两者比较有统计学意义（P〈0、05）；随着肿瘤浸润深度的增高、TNM分期的升高，Id-1的表达阳性率逐渐升高，差异有统计学意义（P〈0．05）。Id-1蛋白的表达与结肠癌组织的分化程度、浸润程度、淋巴结转移、TNM分期有关，与年龄无关。结论Id-1蛋白在结肠癌组织中表达明显升高，并与结肠癌侵袭转移及TNM临床分期相关，可作为结肠癌预后判断的指标之一。Objective To detect the expression of inhibitors of DNA binding-l（Id-1）protein in human colon cancer and investigate the relationship between the expression of Id-1 protein and histological grade, invasion, lymph node metastasis, TNM stage of colon cancer. Methods Immunohistochemistry was used to detect the expression of Id-1 protein in 52 cases of human colon carcinomas, 15 cases of the adjacent normal colon tissues, 16 cases of dysplasia（DYS）, and 17 cases of adenoma. Results The Id-l protein expression was almost not observed in normal colon tissues, and the rate of Id-1 positive expression in adenoma. DYS and colon carcinomas were 29.4%. 43.75%, 73.1%, respectively, which was increased with the lesion progression（P〈 0.05）. Id-1 expression in colon carcinomas with low histological grade was higher than that in carcinomas with higher histological（P〈 0.05）.Positive rate of Id-1 expression in 35 cases with lymph node metastasis was 82.9%（29/35）, which was higher than those without lymph node metastasis（P〈 0.05）. With depth of invasion increasing, stage of TNM upgrading, the positive expression of Id-1 protein in colon carcinomas significantly ascended（P〈0.05）. The rate of Id-1 positive expression was related to invasion, differentiation, metastasis and TNM stage in colon cancer, and no related to age. Conclusion Id-1 might play an important role in the development of invasion and metastasis of colon cancer.

关 键 词：结肠肿瘤 DNA结合抑制蛋白 免疫组织化学 

分 类 号：R735.35[医药卫生—肿瘤]