Fe^(2+)和CO_2对番木瓜ACC氧化酶活性的影响  

Effect of Fe 2+ and CO 2 on ACC Oxidase from Papaya Fruit

作　　者：叶永健[1,2] 宋松泉[1,2] 

机构地区：[1]香港大学植物学系 [2]中山大学生命科学学院

出　　处：《中山大学学报（自然科学版）》1997年第2期18-21,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：香港研究资助局基金

摘　　要：１－氨基环丙烷－１－羧酸（ＡＣＣ）氧化酶催化ＡＣＣ氧化成为乙烯．通过ＤＥＡＥ－Ｓｅｐｈａｒｏｓｅ和Ｐｈｅｎｙｌ－Ｓｅｐｈａｒｏｓｅ柱层析后，ＡＣＣ氧化酶被纯化１９．５倍．在体外分析中，ＡＣＣ氧化酶活性被Ｆｅ２＋和ＣＯ２促进；Ｃｏ２＋、Ｎｉ２＋和Ｍｎ２＋抑制ＡＣＣ氧化酶的活性，但这种抑制效应能部分地被Ｆｅ２＋所拮抗．ＣＯ２对ＡＣＣ氧化酶活性的促进作用与反应体系中氧的浓度有关，在２１％的氧浓度下。Aminocyclopropane 1 carboxylate(ACC)oxidase catalyzes the oxidation of ACC to ethylene. After DEAE Sepharose and Phenyl Sepharose column chromatography, ACC oxidase in the ripe papaya fruit was purified 19.5 fold. The ACC oxidase activity in vitro was enhanced by Fe 2+ and CO 2, and was inhibited by Co 2+ , Ni 2+ and Mn 2+ .The inhibitory effect of Co 2+ , Ni 2+ and Mn 2+ could be partially antagonized by Fe 2+ . The effect of CO 2 on the activity of ACC oxidase was dependent on O 2 concentration in the assay system. The ACC oxidase activity which was increased by CO 2 was higher at 21% O 2 than at 5% O 2 and 1% O 2.

关 键 词：氧化酶 番木瓜 活性 亚铁离子 二氧化碳 

分 类 号：S668.201[农业科学—果树学]