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机构地区:[1]第二军医大学长征医院口腔科,上海200003
出 处:《现代口腔医学杂志》2007年第4期402-405,共4页Journal of Modern Stomatology
摘 要:目的观察咬合力改变对牙周膜成纤维细胞凋亡及其相关基因表达的影响。方法拔除健康雄性SD大鼠右上颌第一、二、三磨牙建立右下颌磨牙咬合力改变动物模型,分别于拔牙后6、12h及1、2、3、5、7、14、28d处死大鼠(n=6),取右下颌磨牙区牙槽骨组织,进行H-E染色和透射电镜观察;免疫组化染色检测牙周膜成纤维细胞Bcl-2和Bax表达。另设正常咬合力大鼠作为对照组(n=6)。免疫组化染色结果采用二级计分法,根据染色强度及阳性细胞的数目进行计分取平均值。结果成功建立咬合力改变SD大鼠模型。H-E染色结果显示实验组牙周膜结构疏松,纤维排列无序,牙槽骨骨壁凹凸不平;透射电镜结果显示实验组牙周膜成纤维细胞出现核固缩、核染色质浓缩、边集。免疫组化染色表明拔牙后12h牙周膜成纤维细胞Bax表达最高(216.83±6.34),而Bcl-2的表达在3d时达峰值(219.33±10.25),此后二者均开始下降,至28d基本恢复正常(7.00±2.37,5.67±2.16,P<0.01)。结论细胞凋亡及其相关基因Bcl-2和Bax参与了牙周膜的改建过程。Objective To study the influence of occlusal force change on apoptosis of periodontal ligament fibroblast (PDLF) and expression of Bcl-2 and Bax in the fibroblasts.Methods Animal model of occlusal force change was established by extracting the right first, second and third maxillary molars in male SD rats. The rats were sacrificed at 6,12 hours and 1, 2, 3, 5, 7, 14, and 28 days after extracting the teeth(n=6), and right mandibular tissues were harvested. Morphologic changes were examined by HE staining and transmission electron microscopy (TEM), apoptosis and its related gene Bcl-2 and Bax expression in PDLF were detected by immunohistochemistry. The rats of normal occlusal forces were used as the control (n=6). The results were assigned a mean score based on the percentage and the intensity of cell positively stained for Bcl-2 and Bax.Results Animal model of occlusal force change was successfully established. HE staining showed that the experiment group had more loose periodontal ligament, disorderly fibres and cells, and uneven alveolar bone than those of the control group. TEM showed that pycnosis and intranuclear chromatin decomposed and coacervated around the edge of nucleus in PDLF. Immunohistochemical staining showed that expression of Bax reached its peak at 12h after extracting the teeth(216.83±6.34), meanwhile, the Bcl-2 expression peaked on day 3 after extraction(219.33±10.25)and then gradually decreased to normal level (7.00±2.37,5.67±2.16,P〈0.01).Conclusion Apoptosis and its related gene Bcl-2 and Bax participate in periodontal ligament remodeling after extracting the teeth.
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