死亡相关蛋白激酶基因在胃癌组织中的表达  

Expression of DAP Kinase in Primary Gastric Cancer

作　　者：叶梅[1] 夏冰[1] 李冬青[2] 周峰[1] 郭秋莎[1] 刘铭球[3] 

机构地区：[1]武汉大学中南医院综合科/消化系病研究中心,湖北武汉430071 [2]武汉大学医学院免疫学系,湖北武汉430071 [3]武汉大学医学院病理学系,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2007年第6期715-718,共4页Medical Journal of Wuhan University

基　　金：湖北省科技攻关项目(编号:2004AA301C91)

摘　　要：目的:研究死亡相关蛋白激酶(DAPk)基因在原发性胃癌组织中的表达,探讨其在胃癌发生发展中可能的作用。方法:收集62例原发性胃癌及相对应的癌旁正常组织。应用RT-PCR检测DAPk mRNA表达,免疫组化方法检测DAPk蛋白表达。结果:RT-PCR方法显示DAPk mRNA表达水平在胃癌组织中明显低于癌旁正常组织(OD值为0.2863±0.2027比0.5736±0.1968,P<0.0001)。免疫组化分析显示,DAPk在所有癌旁正常组织中表达均为阳性,积分范围为2-12,平均8.61±2.89;而在胃癌组织中表达则明显减弱或缺失,积分范围为0-9,平均2.90±3.38。DAPk在癌旁正常组织中的表达水平显著高于胃癌组织(P<0.0001)。结论:在原发性胃癌组织中,DAPk mRNA和蛋白表达明显缺失或低下。Objective： To investigate the expression of death-associated protein kinase（DAPk）, an apoptosis-related serine/threonine kinase gene, in tissue of primary gastric cancer （GC） at mRNA and protein levels. Methods： DAPk mRNA expression was determined by RT-PCR, and the protein expression was measured using immunohistochemical staining in 62 GC tissues and adjacent normal tissues. Results： DAPk mRNA in GC were reduced significantly than in adjacent normal tissues （0. 286±3 0. 202 7 versus 0. 573 6±0. 196 8, P〈0. 000 1）. Immunohistochemical staining revealed that expression of DAPk was significantly lower in GC than in matched normal tissues （2. 90±3.38 versus 8. 61±2.89, P〈0. 000 1）. Conclusion： Expression of DAPk was significantly decreased in gastric cancer at mRNA and protein levels.

关 键 词：死亡相关蛋白激酶 基因表达 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]