骨桥蛋白在肝癌中表达的临床意义  

The clinical significance of expression of osteopontin in hepatocellular carcinoma

作　　者：陆健[1] 李克[1] 卞建民[2] 钱晓峰[3] 蔡永东[2] 姚平[2] 

机构地区：[1]上海交通大学附属第一人民医院普外科,上海200080 [2]南京医科大学附属南京第一医院普外科,江苏南京210006 [3]南京医科大学第一附属医院肝脏外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第9期931-935,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市科技局资助(2003-010-942)

摘　　要：目的:探讨骨桥蛋白(OPN)在肝细胞癌中的表达的临床意义。方法:应用Western blotting法检测21例肝癌及癌旁组织、12例正常肝组织中OPN的表达以及应用免疫组化法对85例肝癌组织、10例肝硬化组织和10例正常肝组织进行OPN、血管内皮生长因子(VEGF)、CD34的检测,用Weidner法计算微血管密度(MVD)。并分析OPN的表达与临床病理特征之间的关系。结果:Western blotting检测结果表明OPN在肝癌组织中的表达显著增高(P<0.01),且OPN高表达和肝癌早期复发转移显著相关(P=0.003)。免疫组化法检测结果表明OPN在肝癌组织中的表达显著高于肝硬化组织和正常组织(P<0.05);且OPN在肝癌组织中的高表达和肿瘤包膜不完整(P=0.03)、感染HBV(P=0.03)和发生早期转移、复发(P=0.018)显著相关;OPN和VEGF在肝癌中的表达呈正相关(r=0.71,P<0.01);OPN在肝癌组织的表达与MVD呈正相关(r=0.44,P<0.01)。结论:OPN在肝癌组织中显著高表达,且OPN与包膜是否完整、感染HBV和早期复发转移相关。OPN和VEGF、MVD的表达显著相关,OPN可能参与了肿瘤血管生成的过程。Objective：To study the clinical significance of osteopontin（OPN） expressions in hepatocellular carcinoma. Methods：Western Blotting was used to detect the expression of OPN in cancerous tissues and paraneoplastic liver tissues from 21 HCC patients. OPN expression was evaluated as well in 12 normal liver tissues. Using immunohistochemistry techniques, the expression of OPN,VEGF and CD34 were measured in 85 surgically removed primary HCCs,10 liver cirrhosis tissues and 10 normal liver tissues. Microvessel density（MVD） was also counted by Weidner method. Furthermore,the correlation between OPN expression and clinicopathologic features was analyzed. Results：The data of Western blotting reveals that OPN was significantly overexpression in HCC and associated closely with early recurrence and metastasis（P = 0.003）.The results from immunohistochemistry demonstrated that OPN in HCC was significantly higher than that in liver cirrhosis and normal tissues（P 〈 0.05）. The OPN was significantly overexpressed in HCC with capsular infiltration（P = 0.03）,HBV（P = 0.03） and associated with early recurrence and metastasis（P = 0.0018）. A positive correlation between OPN and VEGF expressions was found in HCC（r = 0.71,P 〈 0.01）. And there was also a positive correlation between OPN and MVD expressions in hepatocellular carcinioma（r = 0.44,P 〈 0.01）. Conclusion：Overexpression of osteopontin was common in HCC and was significantly correlated with capsular infiltration,HBV and early recurrence and metastasis. The OPN exoression was closely related to VEGF exoression and MVD in HCC,and it indicated that OPN might participate in carcinioma angiogenesis.

关 键 词：肝肿瘤 骨桥蛋白 转移 MVD VEGF 

分 类 号：R735.7[医药卫生—肿瘤]