阿魏酸脂质体的制备及其体外抗氧化活性评价  

Preparation and anti-oxidative activity of liposomal ferulic acid

作　　者：秦晶[1] 陈大为[1] 崔侨[2] 乔明曦[1] 胡海洋[1] 赵秀丽[1] 王薇[1] 

机构地区：[1]沈阳药科大学药学院 [2]沈阳药科大学中日医药研究所,辽宁沈阳110016

出　　处：《药学学报》2007年第10期1092-1096,共5页Acta Pharmaceutica Sinica

摘　　要：采用醋酸钙梯度法制备阿魏酸脂质体,考察不同因素对包封率的影响。制备的脂质体包封率可达到(80.2±5.2)%,粒径约150 nm。空白脂质体和载药脂质体的zeta电位分别为(13.14±1.67)mV和(4.12±0.05)mV。冷冻蚀刻电镜观察结果为单室脂质体。建立了U937细胞株氧化损伤模型,采用MTT法、荧光显微镜及光学显微镜考察了细胞活度、线粒体膜电位及细胞的形态学变化,评价阿魏酸脂质体对U937细胞株氧化损伤的保护作用。结果表明,阿魏酸脂质体组体外抗氧化活性强于阿魏酸溶液。Ferulic acid （FA） was loaded into liposomes via calcium acetate gradient with （80. 2 ± 5.2） % entrapment efficiency. The average sizes of blank liposome and FA liposome were about 155 nm and 154 nm, respectively. The zeta potential of blank liposome and FA liposome were （ 13.14 ± 1.67） mV and （4.12 ± 0.05 ）mV, respectively. Unilamellar vesicles were present in freeze-fracture electron microscopy. In the pharmacodynamic studies, the protective effect of liposomal ferulic acid on tBHP- challenged U937 cells was measured with the morphology of cell injury, mitochondrial transmembrane potential alternation and cell viability assay used as index. The results of MTT assay, microscopy indicated that FA lioosomes exhibited greater antioxidant activity than FA solution on U937 cell.

关 键 词：脂质体 阿魏酸 醋酸钙梯度 MTT 线粒体膜电位 

分 类 号：R943.4[医药卫生—药剂学]