人牙齿切片器官培养模型的建立  

Establishment of human tooth slice organ culture model in vitro

作　　者：朱晓茹[1] 赵守亮[2] 唐荣银[1] 张蓉[1] 李玉成[1] 

机构地区：[1]第四军医大学口腔医学院,西安710032 [2]同济大学口腔医学院

出　　处：《现代口腔医学杂志》2007年第6期600-602,共3页Journal of Modern Stomatology

基　　金：国家自然科学基金资助项目(30471889);第四军医大学后备人才基金资助项目

摘　　要：目的建立人牙齿切片体外器官培养模型。方法收集年轻人健康前磨牙或第三磨牙,用慢速切锯制备2mm厚的牙齿横切片,将切片用含1%琼脂糖的半固体培养基包被后采用Trowel器官培养方法在体外培养2～14天,对培养切片的细胞及组织形态进行组织学观察。结果实验成功的将牙齿切片在体外培养了两周,成牙本质细胞在培养一周之内能够很好的维持原来的形态。一周之后成牙本质细胞逐渐萎缩,细胞密度减小。结论实验成功的建立了人牙齿切片体外器官培养的模型,为后期进行组织损伤和修复、第三期牙本质的形成等方面的研究奠定了基础。Objective To establish human tooth slice organ culture model in vitro. Methods Young healthy human premolars and the third molars were collected, and cut into 2 mm- thick transverse slices by low speed diamondedged saw. The slices were embedded in 1% semisolid agarose- based medium and cultured in Trowel- type cultures for 2 to 14 days. Preservation of cell and tissue morphology was observed. Results Tooth slices were successfully cultured in vitro for two weeks, and the odontoblasts could maintain their original morphology within one week and then gradually shrank and the cell density decreased. Conclusion Human tooth slice organ culture model was successfully established, which provided a better method for further study on dental tissue repair after injury.

关 键 词：人成牙本质细胞 器官培养 牙齿切片 

分 类 号：R78[医药卫生—口腔医学]