腺样囊性癌细胞凋亡抗原负载的树突状细胞诱导抗肿瘤效应的体外研究  

Anti-tumor effect of cytotoxicity T lymphocytes activated by dendritic cells loaded with apoptoic salivary adenoid cystic carcinoma antigen

作　　者：李旭奎[1] 周昌龙[2] 饶国州[1] 张引成[1] 

机构地区：[1]西安交通大学口腔医院口腔颌面外科,西安710004 [2]浙江省宁波市医疗中心李惠利医院口腔科

出　　处：《北京口腔医学》2007年第5期257-260,共4页Beijing Journal of Stomatology

摘　　要：目的探讨负载腮腺腺样囊性癌细胞凋亡肿瘤抗原的树突状细胞,诱导淋巴细胞介导的免疫反应,观察其体外杀伤腺样囊性癌细胞的细胞毒性效应。方法外周血单核细胞,在粒细胞/巨噬细胞集落刺激因子(Granulocyte-macrophage colony-stimulating factor GM-CSF)+白细胞介素-4(Interleukin-4IL-4)的诱导下体外培养,用凋亡肿瘤细胞抗原冲击后,流式细胞仪检测树突状细胞抗原负载前后CD1a、CD83表达量的变化。采用MTT比色法检测同种混合淋巴细胞反应和诱导细胞毒性T细胞(Cytotoxic T lymphocyte CTL)反应。结果凋亡肿瘤抗原刺激后,CD83表达急剧增加(P<0.01),而CD1a表达量下调(P<0.01)。负荷凋亡肿瘤抗原树突状细胞体外诱导出明显的细胞毒性反应,并刺激同种T淋巴细胞增殖。结论GM-CSF+IL-4诱导的单核细胞来源的树突状细胞,能在体外摄取凋亡肿瘤抗原而进一步成熟,通过激活淋巴细胞杀伤癌细胞。Objective To investigate the effect of T lymphocytes activated by dendritic cells （DC） loaded with apoptoic salivary adenoid cystic carcinoma antigen on salivary adenoid cystic carcinoma （SACC）cells. Methods Human peripheral blood monocytes were cultured in RPMI 1640 medium containing 10 % FBS with GM-CSF ＋ IL-4, and continuously incubated with SACC cell lysate made by apoptotic antigen on the day of culture. The expression of DC phenotypes CD1 a and CD83 were evaluated by flow cytometry before and after the pulse. Dendritic cell＇ s function was evaluated by their ability to induce proliferation of allogeneic T cells, and the cytotoxicity of cytotoxic T lymphocyte（ CTL ） activated by DCs loaded with apoptoic antigen evaluated by MTT method. All results were statistically analyzed. Results The expression of CD83 increased after tumor antigen stimulation and CDla was down-regulated. The cytotoxicity against SACC cells was induced by DC loaded with apoptoic antigen, and lymphoeytes proliferation stimulated. Conclusion Dendritic cells generated from monocytes in the presence of GM-CSF and IL-4 conld develop to be mature after tumor antigen pulse. Cytotoxic T lymphocyte activated by dendritic cells loaded with apoptoic antigen had effective cytotoxicity against SACC cells in vitro.

关 键 词：树突状细胞 腺样囊性癌 凋亡肿瘤细胞 

分 类 号：R739.87[医药卫生—肿瘤]