脑源性神经营养因子对缺氧胚脑神经元保护作用的信号传递途径初探  

Signaling Pathway of BDNF Protecting Embryonic Cortical Neurons from Hypoxia

作　　者：汪志凌[1] 毛萌[1] 周晖[1] 李胜富[2] 俞丹[1] 

机构地区：[1]四川大学华西第二医院儿科,成都610041 [2]四川大学华西医院移植免疫与移植工程实验室

出　　处：《四川大学学报（医学版）》2007年第6期934-937,共4页Journal of Sichuan University(Medical Sciences)

基　　金：纽约中华医学会基金(CMB00722);教育部博士点基金(批准号03133)资助

摘　　要：目的研究胚鼠脑皮质神经元遭受缺氧刺激时,脑源性神经营养因子(BDNF)发挥保护作用的信号传递途径。方法采用免疫荧光技术结合激光扫描共聚焦显微镜观察不同缺氧时间点(3h和5h)实验组(分别于缺氧培养前24h和缺氧培养即刻加入BDNF100ng/mL)和对照组(缺氧培养,无BDNF干预)体外培养脑皮质神经元胞内酪氨酸激酶B(TrkB)、磷酸化TrkB和丝裂原活化蛋白激酶(MAPK)水平的变化并进行半定量分析。结果与对照组相比,实验组在缺氧后不同时间点神经元胞浆内TrkB及磷酸化TrkB水平均出现上调(P<0.05),MAPK在胞浆内和核内的表达也增加(P<0.05),且缺氧培养前24h加入BDNF者三种蛋白的表达强于缺氧培养即刻加入BDNF者(P<0.05)。结论BDNF对缺氧神经元的保护作用可能通过诱导TrkB表达上调来实现,以TrkB受体酪氨酸磷酸化起始,经Ras-MAPK途径完成。Objective To investigate the changes of TrkB and Ras-MAPK in brain-derived neurotrophic factor （BDNF） protecting the embryonic rat cerebral cortical neurons against hypoxia-induced neurotoxicity. Methods The immunofluorescence technique, laser scanning confocal microscope and half-quantitative analysis were done to explore the changes of the intracellular levels of tyrosine kinase B （TrkB）, phosphorylated TrkB and the mitogen-activated protein kinase （MARK） in rat embryonic cortical neurons cultured in different time groups of hypoxia with or without BDNF pretreatment. Results The fluorescent intensity of TrkB and phosphorylated TrkB in the cytoplasm and the fluorescent intensity of MAPK in both cytoplasm and cell nucleus of the neurons were significantly increased in the presence of BDNF（P〈 0. 05）, the fluorescent intensity of neurons pretreated with BDNF 24 h before the hypoxia culture was stronger than those with BDNF just before the hypoxia culture （P〈 0. 05）. Conclusion The Ras-MAPK approach may be the major signal transferring way of BDNF in protecting the cortical neurons from hypoxia-induced neurotoxicity. The approach of signal transferring begins with tyrosine phosphorylation of TrkB receptors.

关 键 词：缺氧性脑损伤 脑源性神经营养因子(BDNF) 信号途径 MAPK 

分 类 号：R363[医药卫生—病理学]