缺氧缺糖损伤对未成熟大鼠少突胶质前体细胞表达Nogo-A的影响  

The Influence of Oxygen and Glucose Deprivation on the Expression of Neurite Growth Inhibitor Nogo-A in Oligodendrocyte Precursor Cell of Rat

作　　者：唐军[1] 姚裕家[1] 钟琳[1] 

机构地区：[1]四川大学华西第二医院儿科,成都610041

出　　处：《四川大学学报（医学版）》2007年第6期938-941,共4页Journal of Sichuan University(Medical Sciences)

基　　金：教育部博士点基金项目(编号20050610071)资助

摘　　要：目的观察Nogo-A在新生大鼠少突胶质前体细胞系(OLPs)缺氧缺糖(OGD)损伤后的表达变化,探讨其在OLPs损伤后的作用。方法采用改良振荡分离纯化法体外培养OLPs,特异性抗体A2B5、O4和O1作细胞鉴定;用含连二亚硫酸钠的无糖培养基制作OLPsOGD模型,MTT法检测各组细胞存活率;用免疫荧光法和免疫印迹法观察Nogo-A在细胞OGD后的变化。结果Nogo-A在OLPsOGD10min表达较正常对照组增强,30min继续增高,60min最高,差异有统计学意义(P<0.05);MTT结果显示,OGD30min及60min细胞存活率较对照组降低(P<0.05)。结论OLPs在OGD后Nogo-A表达明显增强,细胞存活率降低,提示Nogo-A可能参与OGD后OLPs的再生抑制过程。Objective To investigate the Nogo-A expression and its regeneration inhibition role in oligodendrocyte precursor cells （OLPs） after oxygen ＆ glucose deprivation （OGD）. Methods The OLPs were separated by the improved procedure of separation and purification through agitation and then cultured in chemically defined medium. The OGD model of OLPs was set up by using Na2S204 and Earle＇s fluid in the medium in vitro, lmmunofluorescence assay was applied to identify the OLPs with its specific antibodies such as A2B5, P4 and O1. Western blot and immunofluorescence assay were used to analyze the expression of Nogo-A in OLPs at 10 rain, 30 rain and 60 rain after OGD. The livahility rate of cells in each group was detected by MTT. Results The expression of Nogo-A was increased significantly compared with control at 10 mln, 30 rain and 60 rain after OGD （P〈0.05）. MTT result showed that the livahility rate of OLPs with OGD was significantly lower than that of control（P〈0. 05）. Conclusion The expression of Nogo-A is increased while the OLPs livahility is dropping down after OGD, which suggests that Nogo-A may play a role in OLPs damage and in the pathogenesis of restraining OLPs＇ regeneration.

关 键 词：Nogo—A 少突胶质前体细胞 缺氧缺糖 

分 类 号：R363[医药卫生—病理学]