木聚糖酶对尼罗罗非鱼钠葡萄糖共转运载体(SGLT_1)mRNA表达的影响  

Effects of the xylanase on sodium/glucose cotransporter 1(SGLT_1) mRNA expression of Oreochromis niloticus

作　　者：聂国兴[1] 王俊丽[1] 华雪铭[2] 黄旭雄[2] 王修启[3] 周洪琪[2] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]上海水产大学生命科学与技术学院,上海200090 [3]华南农业大学动物科学学院,广东广州510642

出　　处：《水产学报》2007年第6期765-770,共6页Journal of Fisheries of China

基　　金：河南省重点科技攻关项目(0423030200);河南省教育厅自然科学研究项目(200510476015);河南省动物学重点学科资助项目

摘　　要：以尼罗罗非鱼[体重(106.16±16.77)g]为实验对象,小麦基础饲料为对照,小麦基础饲料中分别添加不同水平的木聚糖酶(0.05%、0.10%、0.15%)作为试验饲料。每个处理设5个重复,每个重复放养40尾雄性尼罗罗非鱼,旨在研究木聚糖酶对尼罗罗非鱼前肠和中肠钠葡萄糖共转运载体(SGLT_1)mRNA表达的影响,从分子水平揭示木聚糖酶促进尼罗罗非鱼生长的机理。饱食投喂,饲养75 d后,每饲料组分别取10尾鱼,尾静脉取血制备血清,测定血糖含量;采用离心柱型总RNA提取试剂金提取前肠和中肠总RNA,通过RT-PCR对前肠和中肠SGLT_1mRNA的表达进行相对定量。结果表明,0.05%组、0.10%组和0.15%组前肠SGLT_1 mRNA的相对表达量分别比对照组提高19.28%(P<0.05)、42.17%(P<0.01)和16.87% (P<0.05);对照组尼罗罗非鱼在摄食后2 h血糖仅为5.56 mmol·L^(-1),0.10%组极显著高于对照组(P<0.01);0.05%组和0.10%组的增重率较对照组分别提高8.29%、17.45%(P<0.01),0.15%组的增重率与对照组相比没有统计学差异(P>0.05)。研究结果表明,在小麦基础饲料中适量添加木聚糖酶能显著上调前肠SGLT_1mRNA的表达,促进葡萄糖吸收,从而提高尼罗罗非鱼的生长速度。Wheat is important cereal crop in China. Recently, because of the improvement of science andtechnology, the output of wheat has increased significantly and exceeded the demand in phase and structure. Using wheat as raw material of feed, carrying out transformation and consumption by aquaculture, all of these now possess the resources feasibility. The use of wheat in aquatic feed industry as major energy feed has been restricted because of xylan. This paper displayed the mechanism of xylanase promoting animals＇ growth at molecular level. Wheat basal diet was control. The tested diets were wheat basal diet added with different levels of xylanase（0.05%, 0.10%, and 0.15% respectively）. Each treatment was devised with 5 repeats and each repeat had 40 male Oreochromis niloticus. The fish in floating cages were fed to satiation for 75 days. The content of blood glucose in serum of 10 fish from each treat was measured. The total RNA was extracted from the sample＇s fore-gut and mid-gut with spin column total RNA extract kit. The SGLT1 expression was relatively quantified by means of RT-PCR. The results showed that, the xylanase could up- regulate SGLT1 expression of fore-gut, the amount of SGLTl mRNA relative expression of 0.05%, 0.10% and 0.15 % groups had increased by 19.28 % （ P 〈 0.05 ）, 42. 17 % （ P 〈 0.01 ） and 16.87 % （ P 〈 0.05 ） over the control. As for the content of blood glucose, the control group was only 5.56 mmol · L^-1 when fed 2 hours later, the test groups with xylanase supplement were influenced significantly at the same time. The blood glucose content of 0.10% group was higher significantly than that of the control （ P 〈 0.01 ）. The weight gain rate of 0.05% and 0.10% groups were increased by 8.25% and 17.45% respectively （ P 〈0.01 ）, which were higher than that of the control, but it has no significant difference between 0.15% group and the control. So the conclusion is that adding appropriate amount of xylanase to wheat basal diet can significantly up-regulate S

关 键 词：尼罗罗非鱼 小麦基础饲料 木聚糖酶 钠葡萄糖共转运载体1 

分 类 号：S963[农业科学—水产养殖]