SELDI技术应用于肿瘤疗效监测的初步研究  

Application of SELDI-TOF-MS to monitor therapeutic effect on tumors

作　　者：邓碧萍[1] 张辉[1] 贾琳[1] 张秀强[1] 孔北华[1] 曲迅[1] 

机构地区：[1]山东大学齐鲁医院,山东济南250012

出　　处：《山东医药》2007年第30期12-14,共3页Shandong Medical Journal

基　　金：国家自然基金科学基金(30472261);山东省卫生厅科学技术攻关项目(2005HW05)

摘　　要：目的应用表面增强的激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术对卵巢癌及喉癌患者治疗前后血清进行疗效相关标志分子的筛选,评价此技术在肿瘤疗效监测方面的可行性。方法应用SELDI-TOF-MS技术分别对20例卵巢癌患者化疗前后及51例喉癌患者手术前后血清蛋白质指纹图谱进行分析,用Biomarker Wiz- ard软件对结果进行统计学分析。结果卵巢癌患者化疗后血清中质荷比(m/z)4 475的蛋白峰(14/20)较化疗前表达增高;喉癌患者术前血清高表达m/z 13 741蛋白峰,术后21例患者该蛋白表达降低,其中11例(11/21)术后m/z 13 741、m/z 5 192和m/z 15 100的蛋白峰表达均较术前显著下降(P<0.01)。结论SELDI-TOF-MS技术能灵敏的检测肿瘤患者手术及化疗前后血清中的差异蛋白峰。该技术的微创、灵敏、微量等特点将可能在肿瘤疗效监测及肿瘤的个体化治疗研究中具有较好的应用前景。[Objective] To screen bio-marker molecules from the serum of ovarian and laryngeal cancer patients respectively via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry（SEIDI-TOF-MS）. And to by evaluate the feasibility of the method on tumor therapeutic effect monitoring comparing the expression of the biomarkers before and after the clinical intervention. [ Methods ] Serum samples from 20 cases of ovarian cancer patients before and after chemotherapy and 51 cases of laryngeal carcinoma patients before and after operation were analyzed by SEIDI-TOF-MS protein chip. The statistic analysis was done by using Biomarker Wizard software. [ Results ] In ovarian cancer samples, protein peak 4 475 （m/z） was found increased in 14/20 patients after the chemotherapy. The protein peak 13 741 （m/z） was highly expressed in all preoperative patient samples and decreased in 21/51 cases postoperatively. Among this 21 cases, three peaks （13 741,5 192 and 15 100 m/z） were found dramatically decreased in 11 patients after operation（P 〈 0. 01 ）. [ Conclusions] SEIDI-TOF-MS can sensitively detect the change of a protein peak between either pre- and post-operative or pre- and post-chemotherapy, SELDI proteomics technology may have a better foreground on the monitoring of tumor therapeutic effect for its properties of minor injuries, high sensitivity and small sample volume.

关 键 词：卵巢肿瘤 喉肿瘤 表面增强激光解吸电离飞行时间质谱 蛋白质组 疗效监测 

分 类 号：R737[医药卫生—肿瘤]