TGF-β1诱导SUVEC向平滑肌样细胞转分化的初步研究  

Study on TGF-β1 Inducing Transdifferentiation of SUVEC to Smooth Muscle-like Cells

作　　者：王文秀[1] 张彦明[1] 熊奎州[1] 张浩[1] 邓文[1] 代晨[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《上海交通大学学报（农业科学版）》2007年第5期478-482,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家自然科学基金资助项目(No.30471290)

摘　　要：用不同浓度TGF-1β对猪脐静脉血管内皮细胞(SUVEC)进行诱导,研究体外诱导SUVEC向平滑肌细胞分化的可能性。倒置显微镜下观察诱导前后细胞形态的变化,并采用免疫细胞化学SP法对细胞表面特异性标志物进行染色。结果表明,诱导后细胞失去内皮细胞典型的铺路石样形态,而呈现出肌成纤维样细胞;对诱导后的细胞进行免疫组化检测,发现5～10ng.mL-1 TGF-1β诱导后,平滑肌特异性蛋白-αSMA的相对表达量均显著升高;而浓度升高为20 ng.mL-1时,升高不显著;内皮细胞特异性标志(F-ⅧRAg)的表达均显著下降。该研究表明TGF-1β可诱导猪脐静脉血管内皮细胞向肌样细胞分化,但诱导分化的能力与TGF-1β的浓度有关。To demonstrate the possibility of swine umbilical vein vascular endothelial cells （SUVEC） to transdifferentiate into smooth muscle like cells, porcine vascular endothelial cells were incubated with transforming growth factor-β1 on different concentration. The changes in morphology or expression of specific marker of cells were analyzed by Immunohistochemical staining and image analysis method. It showed that expression of α-SMA increased distinctly when treated with 5-10 ng·mL^-1 TGF-β1, whereas expression of F-Ⅷrelated antigen in the cells notably declined. Interestingly, 20ng/mL TGF-β1 did not promote up-regulating of α- SMA. SUVECs lost their cobblestone morphology and converted into ragged cells in TGF-β1-treated culture. We demonstrated here that swine vascular endothelial ceils can be transdifferentiated into smooth muscle like cells treated with TGF-β1. What＇s more, the ability of transdifferentiation correlated to the concentration of TGF-β1.

关 键 词：脐静脉血管内皮细胞 转化生长因子Β1 转分化 平滑肌样细胞 

分 类 号：Q254[生物学—细胞生物学]