根管消毒药物体外细胞毒性和细胞回复能力的研究  

The study of cytotoxicity of different intracanal medications and cell rehabilitation on human periodontal ligament fibroblasts

作　　者：王晓丽[1] 宋萌[1] 娄佳宁[1] 钮晓勇[1] 

机构地区：[1]上海交通大学附属第一人民医院口腔科,上海200080

出　　处：《上海口腔医学》2007年第5期512-519,共8页Shanghai Journal of Stomatology

摘　　要：目的:探讨5种常用根管消毒药物的细胞毒性以及毒性的可逆性,寻找一种较理想的根管消毒药物。方法:用体外培养的人牙周膜成纤维细胞(HPLFs),通过MTT法、ALP活性测定法以及透射电镜等方法,系统评价甲醛甲酚(FC)、樟脑酚(CP)、甲硝唑、氢氧化钙和中药制剂的细胞毒性及其毒性的可逆性。采用SPSS11.5软件包,所有数据均经正态分布检验、方差齐性检验、t检验和方差分析。结果:FC和CP对HPLFs的增殖、ALP活性均有显著抑制作用(P<0.01、P<0.05)。FC的细胞毒性为3～4级,细胞回复度<30%。CP的细胞毒性为1～3级,细胞回复度<30%。甲硝唑对HPLFs的增殖、ALP活性有轻微抑制作用,细胞毒性为1级,细胞回复度为32%～85%。氢氧化钙和中药制剂对HPLFs的增殖和ALP活性均无显著抑制作用(P>0.05),细胞毒性为0～1级,细胞回复度>90%。通过透射电镜观察,HPLFs的超微结构显示,FC、CP有重度毒性,甲硝唑有轻度毒性,而氢氧化钙和中药制剂基本无毒性。结论:FC、CP细胞毒性大,且毒性不可逆;甲硝唑有轻微细胞毒性,但毒性可逆;氢氧化钙和中药制剂无细胞毒性。临床上可用氢氧化钙或中药制剂作为较理想的根管消毒药替代有毒性的FC、CP。PURPOSE： To determine the cytotoxicity and reversibility of cytotoxicity of 5 intracanal medications in various concentrations with human periodontal ligament fibroblasts （HPLFs）,with the aim to provid a comprehensive reliable scientific evidence for making a good choice of these intracanal medications in clinical practice. METHODS： The experimental medications included formocresol （FC）,camphorated paramonochlorophenol （CP）,metronidazole ,calcium hydroxide and traditional Chinese medicine. HPLFs were obtained from healthy premolars extracted for orthodontic reasons. All samples were placed into DMEM ,cultured and harvested from the fourth to eighth passages. MTT, alkalinephosphatase （ALP）activity determination and transmission electron microscope （TEM）were used in this study.The data was analyzed by SPSS11.5 software. Student＇s t test,analysis of variance was used for the statistical analysis. RESULTS：FC,CP had cytotoxicity and inhibited the growth and ALP activity of HPLFs significantly （P〈0.01and P〈0.05）. The cytotoxicity of FC was at 3-4 grade ,with cell rehabilitation rate less than 30%. The cytotoxicity of CP was at 1 to 3 grade, with cell rehabilitation rate less than 30%.Metronidazole lightly inhibited the proliferation and ALP activity of HPLFs, its cytotoxicity was at 1 grade ,with rehabilitation rate from 32% to 85%.Calcium hydroxide and Chinese medicine didn＇t inhibit the proliferation and ALP activity of HPLFs significantly （P〉0.05）.Their cytotoxicity was at 0 to 1 grade with cell rehabilitation rate more than 90%.TEM study showed that severe morphologic alteration of HPLFs induced by FC, CP, whereas the cells in Chinese medicine and calcium hydroxide had little alteration on their morphology. Metronidazole induced minor morphologic alteration of HPLFs. CONCLUSIONS： Calcium hydroxide, traditional Chinese medicine have no cytotoxicity while FC and CP lead to severe cytotoxicity, the cytotoxicity is irreversible .Therefore the clinical application of FC and

关 键 词：根管消毒药 细胞毒性 细胞回复能力 人牙周膜成纤维细胞 

分 类 号：R781.05[医药卫生—口腔医学]