黄皮LEAFY同源基因的克隆与序列分析  

Cloning and Sequence Analysis of LEAFY Homologous Gene from Clausena lansium

作　　者：郭永泽[1] 何新华[1] 李杨瑞[2] 罗聪[1] 

机构地区：[1]广西大学农学院,南宁530004 [2]广西农业科学院广西作物遗传改良与生物技术重点实验室,南宁530007

出　　处：《林业科学》2007年第10期130-133,共4页Scientia Silvae Sinicae

基　　金：广西自然科学基金(桂科自0542022);广西科学基金应用基础研究专项(桂科基0731040)

摘　　要：Clausena lansium, Citrus sinensis and Poncirus trifoliata belong to the different genus of Rutaceae. On the basis of LEAFY (LFY in brief) gene sequences of C. sinensis and P. trifoliata, a pair of 21 bp primers was designed and used to amplify about a 2 300 bp DNA fragment from C. lansium by PCR. The DNA fragment was cloned into pMD18-T vector and then sequenced. The sequence analysis showed that the genomic DNA clone with 2 346 bp contained a complete coding sequence of LFY homologous gene (ClLFY) which 3 exons were composed of a completed open reading frame and encoded 397 amino acids. The identities of nucleotide sequences of LFY genes between C. lansium with C. sinensis and P. trifoliata were more than 92%, and their identities of amino acid sequences were 95%.The result also indicated that C. lansium LFY gene was one amino acid less than the LFY homologous genes from C. sinensis and P. trifoliata.The research laid a sound foundation for studying the regulation and control in flowering mechanism in C. lansium at the molecular level in the future.Clausena lansium, Citrus sinensis and Poncirus trifoliata belong to the different genus of Rutaceae. On the basis of LEAFY （ LFY in brief） gene sequences of C. sinensis and P. trifoliata, a pair of 21 bp primers was designed and used to amplify about a 2 300 bp DNA fragment from C. Iansium by PCR. The DNA fragment was cloned into pMD18-T vector and then sequenced. The sequence analysis showed that the genomic DNA clone with 2 346 bp contained a complete coding sequence of LFY homologous gene （CILFY） which 3 exons were composed of a completed open reading frame and encoded 397 amino acids. The identities of nucleotide sequences of LFY genes between C. lansium with C. sinensis and P. trifoliata were more than 92% , and their identities of amino acid sequences were 95%. The result also indicated that C. lansium LFY gene was one amino acid less than the LFY homologous genes from C. sinensis and P. trifoliata. The research laid a sound foundation for studying the regulation and control in flowering mechanism in C. lansium at the molecular level in the future.

关 键 词：黄皮 LEAFY 克隆 

分 类 号：S666.6[农业科学—果树学] Q943.2[农业科学—园艺学]