水稻种子贮藏蛋白总含量分布和多态性分析  

Total Content and Polymorphism of Seed Storage Proteins in Rice

作　　者：蒋冬花[1] 杨宝峰[1] 叶砚[1] 李杰[1] 黄大年[2] 魏兴华[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]中国水稻研究所水稻生物学国家重点实验室,浙江杭州310006

出　　处：《中国水稻科学》2007年第6期673-676,共4页Chinese Journal of Rice Science

基　　金：中国博士后基金资助项目(2004036170);浙江省科技计划资助项目(2007C32004)

摘　　要：用考马斯亮蓝G-250染色法测定了来自世界各地的110个水稻品种或品系种子贮藏蛋白的含量,发现不同水稻品种或品系种子贮藏蛋白含量存在一定的多样性。110个水稻材料贮藏蛋白含量分布在34.29～95.08mg/g,含量最高的为来自国际水稻研究所的品系C105A51;大部分水稻材料的种子贮藏蛋白含量在40～60mg/g,有72个,占所测水稻材料总数的65.5%;总蛋白质含量超过80mg/g的水稻材料很少,只有3个,占2.7%。SDS-PAGE蛋白电泳图谱表明水稻贮藏蛋白种类和含量存在较高的多态性。Rice seed storage protein contents in 110 rice varieties or lines were determined by using the staining method with Comassie brilliant blue G-250. There were obvious diversities of seed storage protein contents among the varieties or line,,; tested. The contents of rice seed storage proteins ranged from 34.29 to 95.08 mg/g, but 66.3% varieties or lines were in the range of 40 to 60 mg/g. The rice varieties or lines with more than 80 mg/g of rice seed storage protein contents were rare, only accounting for 2.7 % of the total tested materials. The result of SDS-PAGE for seed storage proteins also displayed obvious polymorphisms among the rice varieties or lines. It could provide useful information for seed storage protein analysis and for rice breeding.

关 键 词：水稻 贮藏蛋白 电泳 多态性 

分 类 号：S511[农业科学—作物学]