不同外源激素和培养温度对马铃薯试管薯形成的影响  

Effects of Exogenous Hormones and Temperature on the Formation of Potato Micro-tuber in Vitro

作　　者：蒋从莲[1] 郭华春[1] 

机构地区：[1]云南农业大学薯类作物研究所,云南昆明650201

出　　处：《云南农业大学学报》2007年第6期824-828,共5页Journal of Yunnan Agricultural University

基　　金：云南省科技厅"十一五"攻关项目资助(2006NG08)

摘　　要：以云南栽培的3个马铃薯品种(合作88、大西洋以及转心乌)的脱毒试管苗为材料,研究了不同温度以及不同的外源激素(6-BA,CCC)对试管薯形成的影响。结果表明:以MS+8%蔗糖为基本培养基,在全黑暗条件下,加入CCC比加入BA更有利于诱导结薯。大西洋最适宜的诱导试管苗结薯的培养组合为:CCC 300 mg/L+20℃;合作88为:CCC 500 mg/L+20℃;转心乌则为:CCC 300 mg/L+25℃。Virus -free plantlets of three potato cuhivars （Solanum tubersum L. ） （ Cooperation - 88, Atlantic, Zhuanxinwu） in Yunnan Province were used as the experimental materials. The effects of different combinations of temperature and exogenous hormone（6 -BA and CCC） on the formation of micro-tubers were studied. The results indicated that： the different varieties potatoes required different temperatures and concentration of exogenous hormone. CCC indicated better micro-tubers than BA in full darkness. The optional combinations for Atlantic, Cooperation -88 and Zhuanxinwu were CCC 300 mg/L ＋ 20 ℃, CCC 500 mg/L ＋ 20 ℃ and CCC 300 mg/L ＋ 25 ℃, respectively.

关 键 词：马铃薯 试管薯 矮壮素 6-苄氨基嘌呤 温度 块茎诱导 

分 类 号：S532.01[农业科学—作物学]