固醇调节元件结合蛋白-1c基因多态性与冠状动脉粥样硬化的关系  

Genetic Polymorphism Analysis of SREBP-1c in Patients with Atherosclerotic Cardiovascular Disease

作　　者：徐献群 周新[1] 郑芳[1] 曲喜英[1] 张海燕[1] 

机构地区：[1]武汉大学中南医院基因诊断中心,邮政编码武汉430071

出　　处：《微循环学杂志》2007年第4期59-61,共3页Chinese Journal of Microcirculation

摘　　要：目的:探讨固醇调节元件结合蛋白-1c(SREBP-1c)基因18号外显子54G/C多态性与湖北汉族人冠状动脉粥样硬化(ACD)的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法和基因测序技术,对166例ACD患者(ACD组)和122例健康人(对照组)SREBP-1c基因18号外显子54G/C位点进行分析,并测定血脂水平。结果:SREBP-1c基因18号外显子54G/C在ACD组和对照组中基因型频率分别为CC型6.63%和4.92%,GC型39.76%和28.69%,GG型53.61%和66.39%,差异无显著性意义(P>0.05);ACD组C等位基因频率高于对照组(26.51%vs19.26%,P<0.05)。不同基因型间血脂水平差异均无显著性意义(P>0.05)。结论:等位基因C可能增加ACD发生的风险。Objective: To investigate the association between the polymorphism of sterol regulatory element-binding protein-1c(SREBP-1c) and the level of serum lipid in patients with atherosclerotic cardiovascular diseas (ACD). Method: SREBP-1c genotypes of 166 patients with ACD and 122 healthy controls were detected by polymerase chain reaction and restriction fragment length polymorphism.Meanwhile plasma lipid levels were measured in all subjects. Results: The frequencies of CC,CG and GG were 6.63%,39.76% and 53.61% in ACD group while ones were 4.92%,28.69% and 66.39% in controls. There was not significant difference in frequencies of genotype between controls and ACD cases(χ2=4.751,P>0.05). The frequencies of allele C and G were 26.51% and 73.49% in ACD group,19.26% and 80.74% in controls.There were significant difference in frequencies of allele between controls and ACD cases(χ2=4.112,P=0.043). The gene polymorphism of SREBP-1c had no influence on the serum lipid levels.Conclusion: The C allele of SREBP-1c may be a risk factor of ACD in Han nationality population in Hubei.

关 键 词：固醇调节元件结合蛋白-1C 冠状动脉粥样硬化 基因多态性 PROTEIN 脂代谢紊乱 发病机制 

分 类 号：R587.1[医药卫生—内分泌]