高效液相色谱法检测牛初乳sIgA方法研究  

Research on Determination of Bovine Colostrums sIgA by HPLC

作　　者：刘晓飞[1] 高学军[1] 姚永豪 莫继先[1] 程静[1] 周盛华[3] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030 [2]哈尔滨康普乳品有限公司 [3]中国农业科学院哈尔滨兽医研究所

出　　处：《乳业科学与技术》2007年第6期291-292,295,共3页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：黑龙江省科技厅(GA06B201-4)项目资助

摘　　要：建立高效液相色谱法测定牛初乳sIgA含量。采用TSK-G3000PWxl凝胶色谱柱以0.02mol/L,pH6.8的磷酸盐缓冲液为流动相,流速0.6mL/min,检测波长280nm。结果表明,牛初乳sIgA纯度为52.56%,回收率为74%。说明此方法方便、快捷、准确、可靠、重现性好,可用于牛初乳sIgA含量的检测。Objective to establish HPLC method for the determination of bovine colostrums sIgA. sIgA was separated on TSK-G3000PWxl column with 0.02 mol/L, pH 6.8 PBS, and flow rate was 0.6 mL/min. sIgA was detected at UV 280 nm. Results showed that the purity and yield of sIgA were 52.56% and 74%, respectively. So the toothed is convenient, rapid, accurate, reliable and reproducible. It can be used for the detection of bovine colostrums sIgA.

关 键 词：牛初乳 SIGA HPLC 检测 

分 类 号：TS252[轻工技术与工程—农产品加工及贮藏工程]