不同体积分数洗脱剂对海边月见草茎提取物抗氧化活性的影响  

Effects of Elution Concentration on the Antioxidant Activity of Stem Extract of Oenothera drummondii Hook.

作　　者：陈炳华[1] 谢玉玲[1] 刘剑秋[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2007年第6期86-91,共6页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省自然科学基金资助项目(C0540001)

摘　　要：海边月见草茎提取物经X-5大孔树脂吸附,用体积分数为10%、30%、50%、70%的乙醇溶液进行洗脱,得到4种洗脱级分.采用二苯代苦肼自由基(DPPH.)体系、羟自由基体系、超氧阴离子自由基体系及抗卵黄脂蛋白脂质过氧化体系对4个级分的抗氧化活性进行了测定.结果表明,海边月见草茎提取物4种洗脱级分具有不同程度的清除自由基能力,其中30%乙醇级分的抗氧化活性最强.该级分对DPPH自由基的清除率为82.60%,对H2O2/F e2+产生羟自由基的清除率为65.43%,对邻苯三酚自氧化产生超氧阴离子自由基的抑制率为26.16%,对卵黄脂蛋白PU FA过氧化的抑制率为80.97%.综合评价其抗氧化活性比芦丁好或相当,但比槲皮素略差.同时显示抗氧化活性与其黄酮体积分数有一定的相关性.The crude extract from Oenothera drummondii Hook. stem was adsorbed on macroporous resin X-5, and four fractions were obtained by eluting with 10%, 30%, 50%, 70% ethanol. The antioxidant activity was then characterized by the assays of 1,1- diphenyl -2- picrylhydrazyl （DPPH） radical, hydroxyl radical, superoxide anion radical and polyunsaturated fatty acid （PUFA） from lipoprotein. The results showed that four fractions had different scavenging effects on free radical, while the 30% ethanol fraction did the best, The scavenging or inhibition rate of 30% ethanol fraction to DPPH radical, hydroxyl radical generated by Fenton reaction, superoxide anion radical generated by pyrogallol auto-oxidation system and peroxidation of polyunsaturated fatty acid from lipoprotein were 82.60%, 65.43%, 26.16%, 80.97%, respectively. It showed higher or the same antioxidant activity than Rutin, but lower than Quercetin. In addition, the results also showed that there was a close correlation between antioxidant activity and the concentration of total flavonoids of fractions.

关 键 词：海边月见草 茎提取物 洗脱剂体积分数 不同级分 抗氧化活性 

分 类 号：Q946.91[生物学—植物学] S792.95[农业科学—林木遗传育种]