基于SFE-GC/MS技术的敌敌畏在柳树中的传导与分布  

Determination of Translocation and Distribution of Dichlorvos in Willow Tissue by SFE-GC/MS

作　　者：唐光辉[1] 张璟[1] 何军[1] 冯超[1] 王智辉[1] 张兴[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心

出　　处：《中国农学通报》2007年第10期454-459,共6页Chinese Agricultural Science Bulletin

基　　金：国家创新基金项目"自流式树干注药技术研究"(01C26226100597);陕西省自然科学基金项目"注干药剂在柳树体内传导分布规律及其对天牛的毒杀机理"(2007C113)。

摘　　要：建立了一种利用超临界CO2萃取-气质联用(SFE-GC/MS)技术测定树干注药后柳树中敌敌畏残留量的分析方法。超临界CO2萃取柳树组织中敌敌畏的适宜条件为:温度40℃、压力34.48MPa、静态萃取时间20min、动态萃取CO2体积为20ml(1.0ml/min)、夹带剂甲醇(添加量0.15ml/g)、收集液丙酮。通过上述方法对柳树体内各组织中敌敌畏含量动态变化测定结果表明:敌敌畏经树干注药可在柳树体内经木质部长距离传导,分布于树体各组织中。树干注药后不同时间,敌敌畏在柳树树冠内不同部位的传导、分布存在较大差异。药剂进入木质部后,首先沿注药孔上方输导组织随蒸腾流纵向传导,进入注药孔上方的叶片中。树干注药24h时,树体各部位叶片内药剂含量差异最大,随着时间的延长,药剂在树体内逐渐降解,含量逐渐降低,各部位叶片内药剂含量差异也逐渐缩小,96h后在树冠各部位趋于均衡分布。树干注药初期叶片内药剂含量大于树体其他部位,随时间的延长,含量逐渐下降。注药7d后,药剂在树体各部位趋于均匀分布。Dichlorvos was extracted from willow tissue by supercritical fluid extraction （SFE）. The extraction conditions were extracted at 34.48 MPa and 40℃ with lml/min supercritical carbon dioxide modified with methanol for 20 min after 20-mintue static extraction. The extracts were collected in lOml of acetone. Gas chromatography/mass spectrometry was used to examine the extracts. The results showed that Dichlorvos can be transmitted long distance through stem and distributed in different tissues after trunk injection. The transmission and distribution of Dichlorvos was different in different part of willow crown after trunk injection. Following the transpiration after entered into the stem translation tissue, the pesticide firstly transmitted into the upside leaves over the injection hole. The different maximum appeared at 24 h after trunk injection in different part of crown, then the content declined gradually. The content tended to be balanced in different part of crown at 96 h after trunk injection. The content in leaves was higher than that in other tissues at initial stages after trunk injection and tended to be balanced after 7 d.

关 键 词：超临界流体萃取 气质联用 敌敌畏 树干注药 传导 

分 类 号：S481[农业科学—农药学]