经颅多谱勒检测兔大脑中动脉微血栓栓子的研究  

Experimental study on detection of microthromboembolus of middle cerebral artery in rabbits by transcranial Doppler

作　　者：惠品晶[1] 刘曼[1] 季顺东[2] 付志辉[3] 潘高争[3] 张世明[4] 张学光[1] 惠国桢[1] 

机构地区：[1]苏州大学附属第一医院神经外科,215006 [2]苏州大学附属第一医院血栓室,215006 [3]苏州大学附属第一医院放射科,215006 [4]苏州大学生物技术研究所

出　　处：《中华实验外科杂志》2007年第11期1306-1307,1445,共3页Chinese Journal of Experimental Surgery

摘　　要：目的应用经颅多谱勒(TCD)动态检测兔血栓栓塞模型大脑中动脉(MCA)的微栓子信号(MES),观察栓子的负荷量与缺血性脑血管病(ICVD)发生的相关性。方法将实验动物随机分为3组,即A组含3～5个栓子,B组含6～10个栓子,C组为生理盐水对照组。采用自体动脉血微栓子建立兔局灶性脑缺血模型同时TCD监测MES。栓塞后6 h进行磁共振成像(MRI)及兔脑组织病理观察。结果A、B组TCD均监测到MES;B组MRI的T1WI、T2WI及病理观察见梗死灶,而A组仅在弥散加权成像(DWI)见可疑小缺血灶。结论TCD实时、动态观察微栓子信号,能预测缺血性脑血管病发生的危险性,为临床及早干预治疗提供科学依据。Objective Transcranial Doppler （TCD） was used to dynamically monitor microemboli signals （MES） of middle cerebral artery （MCA） in thrombus embolism models in rabbits and observe the relationship between microemboli signal burdens and ischemic cerebrovascnlar disease. Methods The rabbits were randomly divided into group A （containing 3-5 emboli）, group B （containing 6-10 emboli） and group C （normal saline）. Focal cerebral ischemic models were established by microthromboembolus with autologous arterial blood clots, and MES were monitored by TCD. Magnetic resonance imaging （MRI） was carried and pathological changes were observed 6 h following thrombolysis. Results MES were detected by TCD in groups A and B. The cerebral infarcts were found in T1WI, T2WI of MRI and morphological changes of cerebral infarcts were observed in group B. There was only small suspicion of cerebral ischemic change by diffusion-weighted imag/ng （DWI） in group A. Conclusion TCD could dynamically detect MES and may predict the attack of ischemic cerebrovascular disease, which provided the scientific data to intervene with ischemic cerebrovascular disease earlier.

关 键 词：模型 动物 大脑中动脉 血流动力学 缺血性脑血管病 

分 类 号：R743.3[医药卫生—神经病学与精神病学]