血管紧张素转换酶抑制剂抗胃癌淋巴管生成的研究  

Effects of angiotensin-converting enzyme inhibitors on iymphangiogenesis of gastric cancer in a nude mouse model

作　　者：王亮[1] 何裕隆[1] 张常华[1] 詹文华[1] 蔡世荣[1] 吴晖[1] 彭建军[1] 

机构地区：[1]中山大学附属第一医院胃肠胰外科

出　　处：《中华实验外科杂志》2007年第11期1347-1349,共3页Chinese Journal of Experimental Surgery

基　　金：广东省自然科学基金(5001766)

摘　　要：目的建立人胃癌裸鼠移植瘤模型,观察血管紧张素转换酶抑制剂(ACEI)对胃癌生长和淋巴管生成的影响。方法设立对照组、培哚普利组、卡托普利组,定期观察各组肿瘤生长情况测量肿瘤体积,3周后取出肿瘤标本,采用免疫组织化学测定各组的基质金属蛋白酶(MMP)-7、血管内皮生长因子(VEGF)-C的表达和癌周微淋巴管密度。结果培哚普利组、卡托普利组移植瘤体积与对照组比较明显受到抑制,差异有统计学意义(P<0.01),培哚普利组、卡托普利组肿瘤组织中的MMP-7、VEGF-C(P<0.05)和LMVD与对照组比较均显著降低,差异有统计学意义(P<0.01)。结论卡托普利和培哚普利能明显抑制人胃癌裸鼠移植瘤的生长以及新生淋巴管的形成。Objective To observe the effects of angiotensin-converting enzyme inhibitors （ACEI） on lymphangiogenesis of gastric cancer in a nude mouse model. Methods Nude mouse model of human gastric cancer was established by subcutaneously inoculating human gastric cancer cell line SGC-7901. One week later all 36 mice were randomly divided into 3 groups ： control group, Perindopril group, Captopril group. The mice in 3 groups received the normal saline, Perindopril （2 mg/kg） and Captopril （5 mg/kg） respectively by gavage once a day. The tumor volume was measured regularly. Twenty-one days after treatment the tumors were removed when sections were processed and immunohistochemical method was used to observe the expression of VEGF-C, MMP-7 and lymphatic microvessel density （LMVD）. Results In Captopril and Perindopril groups, tumor volume was significantly inhibited as compared with control group （ both P 〈0.01 ）. In Captopril and Perindopril groups ,the expression of VEGF-C and MMP-7 was both significantly decreased as compared with control group （ both P 〈 0.05）. LMVD in Captopril and Perindopril groups also significantly lower than in the control group （ both P 〈 0.01 ）. Conclusion ACEI may inhibit the growth of gastric cancer model and lymphangiogenesis by inhibiting the expression of MMP-7 and VEGF-C.

关 键 词：胃癌 血管内皮生长因子 血管紧张素转换酶抑制剂 

分 类 号：R735.2[医药卫生—肿瘤]