肿瘤患者PBMC及肿瘤标本IL-4及IL-10检测  

Expression of IL-4 and IL-10 by PBMC and Tumor Tissues in Cancer Patients

作　　者：遇珑[1] 吴易元[1] 惠依群[1] 袁维华[1] 马强中[1] 张友会[1] 韩嘉珠[1] 秦德兴[1] 吴雪林[1] 黄一容[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所,北京100021

出　　处：《中国医学科学院学报》1997年第3期227-231,共5页Acta Academiae Medicinae Sinicae

摘　　要：采用生物活性测定和原位杂交法，检测肿瘤患者的单个核细胞（PBMC）、癌性腹水及肿瘤组织中IL－4及IL－10的活性和表达。淋巴瘤和鼻咽癌患者的PBMC分泌IL－4、IL－2及IFNγ活性低下，而IL－10增高。卵巢癌腹水中IL－10的阳性率为846％，但IL－4均为阴性。用原位杂交法检测胃癌、食管癌及乳腺癌肿瘤组织中IL－4及IL－10的表达，结果两种因子均可在3种瘤组织表达，两者阳性率无明显差别，波动在40％～7O％。IL－10在PBMC、癌性腹水及瘤细胞中活跃表达，提示在某些肿瘤患者，除了上调正向因子外，尚需控制负向因子IL－10的过度分泌。Using biological activity assay, the production of IL-4 and IL-10 by peripheral blood mononuclear cells (PBMC) was studied in patients with nasopharyngeal carcinoma and lym-phoma. The IL-2, IL-4 and IFNγ levels in both cancer patients were lower than that in nor-mal subjects' In contrast, the IL-10 level was higher in both cancer patients' When rIL-2 was added into the cytokine production system, IL-2, IL-4, and IFNγ level were upregulated in lymphoma patients. However, in the patients of nasopharyneal carcinoma, only IL-2 level was upregulated. IL-10 activity could not be affected by exogenous IL-2 in both cancer pa-tients' The results of negative IL-4 activity and higher positive percentage (84. 6%) of IL-10 activity in ascites from patients with ovarian carcinoma were corresponded to the patients with nasopharyngeal carcinoma and lymphoma. By using in situ hybridization technique, the expression of IL-4 and IL-10 was studied in tumor tissues from patients with gastric, esophageal and breast carcinomas. There was no differences of the percentage of IL-4 and IL-10 expression among three kinds of tumor tissues, the range of positive percentage was 4o% ~7o%. These results indicate that the correction of abnormal upregulation of IL-10 ac-tivity should be considered for cancer immunotherapy.

关 键 词：白细胞介素4 白细胞介素10 单个核细胞 肿瘤 

分 类 号：R730.3[医药卫生—肿瘤]