RP-HPLC法测定川芎愈伤组织中阿魏酸的含量  

Determination of Ferulic Acid in Callus of Ligusticum Chuanxiong by HPLC

作　　者：张蓓蕾[1] 夏醒醒[1] 陈勤[1] 

机构地区：[1]安徽大学生命科学学院安徽省中药研究与开发重点实验室,安徽合肥230039

出　　处：《中国中医药信息杂志》2007年第11期43-44,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：安徽省教育厅自然科学基金资助(2005KJ150)

摘　　要：目的建立高效液相色谱法测定川芎愈伤组织中阿魏酸含量的方法。方法利用ZORBAX300SB–C18(250mm×4.6mm,5μm)色谱柱,乙腈-1%三乙胺(15∶85)为流动相,检测波长为320nm,流速1.0mL/min,柱温为27℃,进样量20μL。结果阿魏酸在0.93～4.67μg/mL范围内线性关系良好,r=0.9998,平均加样回收率为99.98%,RSD=1.63%。结论该方法快速简便、准确可靠,可用于川芎愈伤组织中阿魏酸的含量测定。Objective To establish a HPLC method for the determination of Ferulic acid in callus of Ligusticum chuanxiong. Methods The separation was performed on ZORBAX 300SB-C18 （250 mm × 4.6 mm, 5 μm） column, using acetonitrile-1% triethylamine （15 ： 85） as mobile phase, detected at 320 nm. The flow rate was 1.0 mL/min, the column temperature was 27 ℃. Resuits The linear range of Ferulic acid was 0.93～4.67 μg/mL, r=0.999 8, the average recovery was 99.98% and RSD was 1.63%. Conslusion The method was rapid, simple, accurate and stability, and can be used for the quanfitiative determination of Ferulic acid in Ligusficum chuanxiong callus.

关 键 词：川芎 愈伤组织 阿魏酸 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]