耐高温酵母利用稻草粉同步糖化发酵的初步研究  

Primary Study on Simultaneous Saccharifiction and Fermentation of High-temperture Tolerance Yeast and Straw Powder

作　　者：刘海臣[1] 钱甜甜[1] 王玉慧[1] 赵连连[1] 刘敏[1] 阮时雷[1] 吴国光[1] 

机构地区：[1]中国矿业大学化工学院,江苏徐州221116

出　　处：《酿酒科技》2007年第11期25-28,共4页Liquor-Making Science & Technology

基　　金：中国矿业大学大学生科研训练计划项目(No.060607)。

摘　　要：研究不同预处理方法对稻草粉纤维素糖化率的影响。结果表明,冰冻处理可使纤维素酶对稻草粉的水解率提高11.2%;4%NaOH处理稻草粉,料水比1∶10,pH4.8,50℃振荡酶解1h得到还原糖浓度563.49μg/mL,是未处理的17倍;稀碱和机械粉碎对稻草粉预处理具有协同效果;发酵基质料水比8∶100,发酵温度40℃,接种量5%,酶加量25u/g时,产乙醇能力较高。The effects of different pretreatment methods on straw powder saccharification yield were investigated.The results showed freezing treatment could increase hydrolysis rate of straw powder （hydrolyzed by cellulase） by 11.2 %; reducing suger concentration could reach 563.49 μg/mL （17 times as non-pretreatment＇s） under the conditions of 4 % NaOH treatment,the ratio of feed and liquid as 1：10, pH value as 4.8, and oscillation reacting for 1 h at 50℃ ; both aqueous alkali and mechanical grinding treatment could collaboratively increase the saccharification yield; and ethanol production capacityreached the highest as the ratio of materials and water in fermenting substrate as 8：100, fermentation temperature at 40 ℃, inoculation quantity as 5 %, and the addition level ofcellulase as 25 u/g.

关 键 词：乙醇 稻草粉 耐高温酵母LZ2 同步糖化发酵 

分 类 号：TS261[轻工技术与工程—发酵工程]