高效液相色谱法测定活血止痛胶囊中阿魏酸含量  

Determination of Ferulic Acid in Huoxuezhitong Capsule by HPLC

作　　者：王明江 

机构地区：[1]浙江省舟山存德医药有限公司,浙江定海316000

出　　处：《中国药业》2007年第22期38-39,共2页China Pharmaceuticals

摘　　要：目的建立测定活血止痛胶囊中阿魏酸含量的高效液相色谱(HPLC)法。方法采用Diamonsil TMC18色谱柱(250mm×4.6mm,5μm),以甲醇-乙腈-2%冰乙酸溶液(20∶10∶70)为流动相,检测波长为322nm。结果阿魏酸质量浓度在2.78～55.6μg/mL之间与峰面积线性关系良好(r=0.9997,n=5),方法平均回收率为99.21%,RSD=0.35%(n=6)。结论HPLC法操作简便、快速、准确,可用于活血止痛胶囊的含量测定。Objective To develop a HPLC method for the determination of ferulic acid in Huoxuezhitong Capsule, Methods Diamonsil^TMC18 column was used, The mobile phase was methanol -acetonitrile - 2% glacial acetic acid（20 ： 10 ： 70）. The detection wavelength was 322 nm, Results The linear range for it was 2. 78 - 55. 60 μg/mL（ r = 0. 999 7, n = 5）. The average recovery rate was 99. 21% , RSD = 0.35% （ n = 6）. Conclusion The method is simple, rapide and accurate, It can be used for the determination of ferulic acid in Huoxuexhitong Capsule.

关 键 词：活血止痛胶囊 阿魏酸 高效液相色谱法 含量测定 

分 类 号：R286.0[医药卫生—中药学] R284.1[医药卫生—中医学]