川芎嗪对严重烧伤大鼠心肌细胞凋亡的影响  

Effects of Tetramethylpyraxine on Myocardial Apoptosis in Severely Burned Rats

作　　者：刘丽乔[1] 章洁[1] 杨晓红[1] 李国辉[2] 万福生[1] 

机构地区：[1]南昌大学医学院生物化学与分子生物学教研室,南昌330006 [2]南昌大学第一附属医院烧伤中心,南昌330006

出　　处：《江西医学院学报》2007年第5期9-13,共5页Acta Academiae Medicinae Jiangxi

基　　金：江西省自然科学基金(0040054)

摘　　要：目的:观察川芎嗪(TMP)对烧伤后大鼠心肌细胞凋亡的影响及初步机制探讨。方法选健康Wistar大鼠50只,随机分为5组,对照组、烧伤组(即B组,造成30%TBSAⅢ度烧伤)、烧伤+TMP组(B+TMP组,烫伤后即刻腹腔注射TMP,20 mg/kg)、烧伤+SB202190组(B+SB组,烫伤后立即注射SB202190,10 mg/kg)及烧伤+TMP+SB202190组(B+TMP+SB组)。放射免疫法测定TNFα含量,末端标记(TUNEL)法检测凋亡细胞、RT-PCR,免疫组化分析心肌Fas、caspase-8、Bcl-2和Bax基因表达,荧光分析法测定Caspase-3活性。结果与对照组比较,B组大鼠心肌细胞凋亡指数(17.46±2.45),心肌中TNFα含量,Fas、caspase-8和Bax蛋白水平及caspase-3活性均显著性升高,但Bcl-2蛋白表达显著低于对照组(P<0.01)。B+TMP组大鼠心肌TNFα含量、心肌细胞凋亡指数(4.15±0.41),心肌Fas、caspase-8和Bax表达水平及caspase-3活性均显著性低于B组,而Bcl-2蛋白表达水平则显著高于B组(P<0.01)。结论TMP对烧伤后心肌细胞凋亡有较好的抑制作用,其机制可能是TMP通过降低TNFα的生成及调节凋亡相关基因的表达。Objictive To investigate the effects of tetramethylpyraxine（TMP）on myocardial apoptosis following severe burns. Methods A total of 50 healthy adult Wistar rats were randomly divided into 5 groups ： The control group （n = 10, without burns）, the burn group （n= 10, subjected to a 30% TBSA Ⅲ degree scald injury）;the TMP treatment group（n=10,treated by intrapleural injection tetramethylpyraxine （20 mg/kg） after scald injury immediately） ; the TMP ＋ SB202190 treatment group（n= 10）treated by intrapleural injection tetramethylpyraxine （20 mg/kg） and SB202190（10 mg/kg）, and SB202190 treatment group（n = 10）treated by intrapleural injection SB202190（10 mg/kg）. Levels of tumor necrosis factor alpha（TNFα）in plasma and in myocardium were assayed at 18 post burn hours. Terminal deoxynucleotidy transferase-mediatde dUTP fluoescein nick end labeling（TUNEL） and S-P immunohistochemical staining were used respectively to determine the apoptosis and gene expressions of Fas, caspase-8, Bcl-2 and Bax. The caspase-3 activity was detected by Fluorospectrophotometry. Results Apoptosis index（17.46±2.45） ,TNFα contents, levels of fas, caspase-8 ,Bax gene expression and caspase-3 activity in the rat＇s myocardium of the burn group were all higher than that of the control group,and the expression of Bcl-2 protein in the myocardium of the burn group was significantly lower than that of the control group（P〈0.01） ;In the TMP treatment group,Apoptosis index（4.15±0.41） ,TNFα contents in plasma and myocardium,levels of fas,caspase-8 and bax gene expression and caspase-3 activity in the myocardium were lower than that of the burn group significantly,the expression of Bcl-2 protein in the rat＇s myocardium was higher than that of the burn group significantly（P〈0.01）. Conclusion Tetramethylpyraxine exerts a good antagonist action on the myocardial apoptosis following severe burns,it could inhibit the production of TNFα and regulate the gene expression of fas, caspase-8,

关 键 词：川芎嗪 凋亡 烧伤 基因表达 信号转导 心肌 大鼠 

分 类 号：R-332[医药卫生]