染料浓度和盐度对白腐真菌同工酶的电泳分析  

Effect of Dye and Salt Concentration on Peroxidase Isoenzyme Pattern of White Rot Fungi

作　　者：武琳慧[1] 黄民生[1] 施华宏[1] 

机构地区：[1]华东师范大学资源与环境科学学院,上海200062

出　　处：《环境科学与技术》2007年第11期7-9,共3页Environmental Science & Technology

基　　金：国家自然科学基金资助课题(50278034)

摘　　要：应用聚丙烯酰胺凝胶电泳技术分析,并比较了染料废水的两大因子(染料浓度和盐度)对黄孢原毛平革菌过氧化物同工酶谱带的影响程度,结果表明:在染料或盐胁迫下,黄孢原毛平革菌均表现出应急产酶的响应,虽然过氧化物同工酶谱带数量未发生增加或减少,但谱带强度受到较大影响,活性艳红X-3B浓度≤200mg/L或NaCl浓度≤15g/L时均能诱导过量产酶,继续提高染料浓度或盐度将导致产酶抑制,染料浓度对酶带的影响作用高于盐度。Isoenzyme patterns of peroxidase of Phanerochaete chrysosporium under different dye or salt concentration were studied. Lower concentration of reactive brilliant red X-3B or NaCl could obviously induced over-production of peroxidase, whereas high concentration of reactive brilliant red X-3B less than 200mg/L had greatly inhibitive effect on peroxidase production. Results indicated that Phanerochaete chrysosorium had undergone a strong response for peroxidase production to dye and salt concentrations, that was inducement, inhibition and exhaustion.

关 键 词：白腐真菌 染料 氯化钠 浓度 同工酶谱带 

分 类 号：X172[环境科学与工程—环境科学]