右旋氨基酸氧化酶在胃癌和正常胃黏膜细胞中的表达  

The expression of DAO in gastric cancer cells and gastric mucosa cells

作　　者：刘冀红[1] 曹伟新[1] 蔡劬[2] 刘炳亚[2] 朱正纲[3] 林言箴[3] 

机构地区：[1]上海交通大学附属瑞金医院临床营养科,上海200025 [2]上海交通大学附属瑞金医院上海消化外科研究所,上海200025 [3]上海交通大学附属瑞金医院普通外科,上海200025

出　　处：《肠外与肠内营养》2007年第3期149-152,共4页Parenteral & Enteral Nutrition

摘　　要：目的:研究右旋氨基酸氧化酶(DAO)在胃癌和正常胃黏膜细胞中的表达。方法:以裸鼠肝、肾组织为对照,采用定量PCR法,检测7株胃癌细胞和胃黏膜细胞株GES-1中DAO的表达。用RT-PCR法检测46份新鲜胃癌组织和正常胃黏膜组织标本中DAO的表达。结果:DAO在裸鼠肝和肾组织中有表达,在肾组织中表达量较高;7株胃癌细胞和正常胃黏膜细胞株GES-1中均无DAO表达;除1份胃癌组织标本中DAO有表达外,其余胃癌组织和正常胃黏膜组织中均无DAO表达。结论:胃癌细胞和正常胃黏膜细胞中均无DAO表达,右旋甲硫氨酸替代左旋甲硫氨酸用于胃癌的营养治疗,可避免甲硫氨酸饥饿对肝、肾等重要器官的不利影响。Objective：To study the expression of D-amino acid oxidase （DAO） in gastric cancer ceils and gastric mucosa cells. Methods ：The expression of DAO was detected in seven gastric cancer cell lines and a normal gastric mucosa cell line GES-1 by real-time quantitative PCR and in tumor tissues and normal gastric mucous tissues of 46 patients with gastric cancer by RT-PCR. The liver and kidney tissues of nude mouse acted as positive controls. Results：DAO was expressed in the liver and kidney tissues of nude mouse, and DAO expression in kidney tissue was higher than that in liver tissue. DAO was not detected in seven gastric cancer cell lines and the normal gastric mucosa cell line GES-1. Except one tumor tissue sample, DAO was not detected in other gastric cancer tissues and all normal mucosa tissues. Concluslon：DAO was not ex- pressed in gastric cancer cells and gastric mucosa cells. The nutritional treatment of D-Met in place of L-Met could avoid the disadvantage of methionine starvation to important organs, such as liver and kidney.

关 键 词：右旋氨基酸氧化酶 胃癌 右旋甲硫氨酸 

分 类 号：R735.2[医药卫生—肿瘤]