软骨多糖抑制Hca-F肝癌细胞转移的初步研究  

Experimental Study of the Inhibitory Effect on the Metastasis of Hca-F Hepatocyte by Cartilage Polysaccharide

作　　者：刘安军[1] 刘艳玲[1] 张国蓉[1] 胡延勋[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《现代生物医学进展》2007年第11期1649-1650,1634,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(207761137);天津科技大学人才引进及科研启动基金(0200038)

摘　　要：目的:研究软骨多糖抑制小鼠淋巴结高转移肝癌细胞Hca-F侵袭转移的作用。方法:以615近交系小鼠右脚垫皮下注射肝癌细胞Hca-F建立转移模型,设模型组和给药组。检测软骨多糖抑瘤率、淋巴道转移抑制率、淋巴结石蜡切片HE染色观察肿瘤细胞侵袭情况和采用ELISA试剂盒测定血清中TNF-α的含量。结果:软骨多糖具有明显的抑瘤作用,抑瘤率达61.9%;淋巴结转移抑制率为22.2%;通过比较模型组和给药组瘤细胞转移部位淋巴结的HE染色,发现给药组转移情况明显低于模型组;ELISA检测表明给药组血清中TNF-α的含量明显低于模型组(P<0.05)。结论:软骨多糖一定程度上能够抑制肝癌细胞Hca=F向周围淋巴结转移,为开发治疗恶性肿瘤的药物提供了选择。Objective： To study the inhibitory effect of cartilage polysaccharide on the lymphatic metastasis of Hca-F hepatocyte in mice. Methods： Mouse model was established by injecting ascetic hepatoma Hca-F cells into their right axilla, 20 mice were divided into two groups. Rats in the model group were injected with saline i.p. and in the treatment group were injected with cartilage polysaccharide i.p.. The inhibitory effect of cartilage polysaccharide was analyzed by measuring the tumor-inhibition rate and the lymphatic metastasis inhibition rate. Metastasis of hepatocellular carcinoma was observed by lymph node HE staining and the content of serum TNF-α was detected by ELISA assay. Results： Cartilage polysaccharide has significant inhibition effect on tumor growth. Tumor-inhibition rate in this study is 61.9%, and the lymphatic metastasis inhibition rate is 22.2%. Compared with the model group, the HE staining of tumor metastasis lymph node showed that the lymphatic metastasis degree of treated group was lower. The content of serum TNF-α in treated group was lower than the model group by ELISA assay （P〈0.05）. Conclusion： Cartilage polysaccharide can inhibit the metastasis of Hca-F hepatocyte to nearby lymph nodes, and it is a potential choice for anti-tumor drug development.

关 键 词：软骨多糖 肿瘤转移 抑瘤率 TNF-Α 

分 类 号：R735.7[医药卫生—肿瘤]